PMID- 6309410
OWN - NLM
STAT- MEDLINE
DCOM- 19831008
LR  - 20220311
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 34
IP  - 1
DP  - 1983 Aug
TI  - Structures of P transposable elements and their sites of insertion and excision 
      in the Drosophila melanogaster genome.
PG  - 25-35
AB  - We have isolated and characterized several members of the P transposable element 
      family from a Drosophila melanogaster P strain. Large 2.9 kb elements are present 
      as multiple highly conserved copies together with smaller (0.5-1.6 kb), 
      heterogeneous elements. The complete DNA sequences of the 2.9 kb element and four 
      small elements (previously isolated from hybrid-dysgenesis-induced mutations of 
      the white locus) have been determined. Each small element appears to have arisen 
      from the 2.9 kb element by a different internal deletion. P elements have 31 bp 
      perfect inverse terminal repeats and upon insertion duplicate an 8 bp sequence 
      found only once at the site of insertion. Three of the insertions into the white 
      locus occurred at the same nucleotide, indicating a high degree of local site 
      specificity for insertion. The basis of this specificity has been investigated by 
      DNA sequence analysis of the sites where 18 P elements are found. A revertant of 
      one of the white locus mutants has been found to result from precise excision of 
      the P element, restoring the wild-type DNA sequence.
FAU - O'Hare, K
AU  - O'Hare K
FAU - Rubin, G M
AU  - Rubin GM
LA  - eng
SI  - GENBANK/V01520
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA Transposable Elements)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - *DNA Transposable Elements
MH  - Drosophila melanogaster/*genetics
MH  - Embryo, Nonmammalian/physiology
MH  - *Genes
MH  - Mutation
MH  - *Recombination, Genetic
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 1983/08/01 00:00
MHDA- 1983/08/01 00:01
CRDT- 1983/08/01 00:00
PHST- 1983/08/01 00:00 [pubmed]
PHST- 1983/08/01 00:01 [medline]
PHST- 1983/08/01 00:00 [entrez]
AID - 0092-8674(83)90133-2 [pii]
AID - 10.1016/0092-8674(83)90133-2 [doi]
PST - ppublish
SO  - Cell. 1983 Aug;34(1):25-35. doi: 10.1016/0092-8674(83)90133-2.