PMID- 6319165
OWN - NLM
STAT- MEDLINE
DCOM- 19840305
LR  - 20190707
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 150
IP  - 2
DP  - 1984 Feb
TI  - Effect of an anti-murine transferrin receptor-ricin A conjugate on bone marrow 
      stem and progenitor cells treated in vitro.
PG  - 400-7
AB  - A monoclonal antibody with specificity for murine transferrin receptor was 
      conjugated with the toxic A subunit of ricin. The dose range, specificity, and 
      kinetics of inhibition of protein synthesis of the conjugate were determined on 
      the murine T-lymphoma cell line, BW5147. When toxin was present throughout the 
      period of culture, in vitro myeloid (CFUc) and erythroid (CFUe and BFUe) bone 
      marrow colonies were inhibited by doses of conjugate comparable to those that 
      inhibit protein synthesis in murine cell lines (IC50 of 5 X 10(-11)M). Bone 
      marrow exposed briefly (30 min to 6 h) to anti-transferrin receptor 
      antibody-ricin A conjugate was assayed for myeloid (CFUc) and erythroid (CFUe and 
      BFUe) progenitors in vitro and for in vivo spleen colony formation (CFUs). Only 
      CFUe were depleted by this pulse exposure, consistent with the higher frequency 
      of proliferating cells and transferrin receptor expression in the CFUe population 
      relative to other progenitors.
FAU - Lesley, J
AU  - Lesley J
FAU - Domingo, D L
AU  - Domingo DL
FAU - Schulte, R
AU  - Schulte R
FAU - Trowbridge, I S
AU  - Trowbridge IS
LA  - eng
GR  - CA-17733/CA/NCI NIH HHS/United States
GR  - CA-25893/CA/NCI NIH HHS/United States
GR  - CA-34787/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Transferrin)
RN  - 9009-86-3 (Ricin)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Cell Division
MH  - Cell Line
MH  - Colony-Forming Units Assay
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - Leukemia
MH  - Lymphoma
MH  - Mice
MH  - Receptors, Cell Surface/analysis/immunology/*physiology
MH  - Receptors, Transferrin
MH  - Ricin
MH  - Spleen/cytology
MH  - T-Lymphocytes
EDAT- 1984/02/01 00:00
MHDA- 1984/02/01 00:01
CRDT- 1984/02/01 00:00
PHST- 1984/02/01 00:00 [pubmed]
PHST- 1984/02/01 00:01 [medline]
PHST- 1984/02/01 00:00 [entrez]
AID - 10.1016/0014-4827(84)90583-4 [doi]
PST - ppublish
SO  - Exp Cell Res. 1984 Feb;150(2):400-7. doi: 10.1016/0014-4827(84)90583-4.