PMID- 6339421
OWN - NLM
STAT- MEDLINE
DCOM- 19830505
LR  - 20220408
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 31
IP  - 1
DP  - 1983 Jan 15
TI  - Production of a mouse monoclonal antibody reactive with a human nuclear antigen 
      associated with cell proliferation.
PG  - 13-20
AB  - The production of a mouse monoclonal antibody, Ki-67, is described. The Ki-67 
      antibody recognized a nuclear antigen present in proliferating cells, but absent 
      in resting cells. Immunostainings with Ki-67 revealed nuclear reactivity in cells 
      of germinal centres of cortical follicles, cortical thymocytes, neck cells of 
      gastrointestinal mucosa, undifferentiated spermatogonia and cells of a number of 
      human cell lines. The Ki-67 antibody did not react with cells known to be in a 
      resting stage, such as lymphocytes, monocytes, parietal cells and Paneth's cells 
      of gastrointestinal mucosa, hepatocytes, renal cells, mature sperm cells, brain 
      cells, etc. Expression of the antigen recognized by Ki-67 could be induced in 
      peripheral blood lymphocytes after stimulation with phytohaemagglutinin, whereas 
      it disappeared from HL-60 cells stimulated with phorbol esters to differentiate 
      into mature macrophages in a resting stage. These findings suggest that Ki-67 is 
      directed against a nuclear antigen associated with cell proliferation. A first 
      series of immunostainings of tumour biopsies indicated that Ki-67 may be a potent 
      tool for easy and quick evaluation of the proportion of proliferating cells in a 
      tumour.
FAU - Gerdes, J
AU  - Gerdes J
FAU - Schwab, U
AU  - Schwab U
FAU - Lemke, H
AU  - Lemke H
FAU - Stein, H
AU  - Stein H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens)
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Nucleoproteins)
RN  - 0 (Phytohemagglutinins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibody Specificity
MH  - Antigens/*immunology
MH  - Antigens, Nuclear
MH  - *Cell Division
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Lymphocyte Activation
MH  - Lymphocytes/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Monocytes/immunology
MH  - Nucleoproteins/*immunology
MH  - Palatine Tonsil/immunology
MH  - Phytohemagglutinins/pharmacology
MH  - Thymus Gland/immunology
EDAT- 1983/01/15 00:00
MHDA- 1983/01/15 00:01
CRDT- 1983/01/15 00:00
PHST- 1983/01/15 00:00 [pubmed]
PHST- 1983/01/15 00:01 [medline]
PHST- 1983/01/15 00:00 [entrez]
AID - 10.1002/ijc.2910310104 [doi]
PST - ppublish
SO  - Int J Cancer. 1983 Jan 15;31(1):13-20. doi: 10.1002/ijc.2910310104.