PMID- 6373002
OWN - NLM
STAT- MEDLINE
DCOM- 19840720
LR  - 20061115
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 44
IP  - 7
DP  - 1984 Jul
TI  - Demonstration of an extracellular matrix in multicellular tumor spheroids.
PG  - 3090-7
AB  - Multicellular spheroids of a human glioma cell line (U-118 MG) and a human 
      thyroid cancer cell line ( HTh -7) were analyzed for the presence of 
      extracellular matrix (ECM) using light microscopy, transmission electron 
      microscopy, and indirect immunofluorescence staining for fibronectin, laminin, 
      and collagen. These studies were supplemented by analyses of glycosaminoglycans 
      using autoradiography or chemical methods after metabolic labeling with 
      [35S]sulfate or [3H]glucosamine in conjunction with various extraction 
      procedures. The results showed that both types of spheroids contained an ECM 
      composed of fibronectin, laminin, collagen, and glycosaminoglycans. The 
      organization of the ECM in the spheroids seemed to be similar to that of tumors 
      in vivo. These findings help justify the use of the spheroid system as an in 
      vitro model for the study of biological phenomena of human tumors in vivo. 
      Furthermore, it is concluded that the formation of an ECM in vitro is not 
      confined to normal cells but can be promoted in transformed cells using 
      appropriate culture conditions.
FAU - Nederman, T
AU  - Nederman T
FAU - Norling, B
AU  - Norling B
FAU - Glimelius, B
AU  - Glimelius B
FAU - Carlsson, J
AU  - Carlsson J
FAU - Brunk, U
AU  - Brunk U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Fibronectins)
RN  - 0 (Laminin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Cell Line
MH  - Collagen/analysis
MH  - Fibronectins/analysis
MH  - Fluorescent Antibody Technique
MH  - Glioma/*pathology/ultrastructure
MH  - Humans
MH  - Laminin/analysis
MH  - Microscopy, Electron
MH  - Thyroid Neoplasms/*pathology/ultrastructure
EDAT- 1984/07/01 00:00
MHDA- 1984/07/01 00:01
CRDT- 1984/07/01 00:00
PHST- 1984/07/01 00:00 [pubmed]
PHST- 1984/07/01 00:01 [medline]
PHST- 1984/07/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1984 Jul;44(7):3090-7.