PMID- 6465851
OWN - NLM
STAT- MEDLINE
DCOM- 19840824
LR  - 20161123
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 4
IP  - 3
DP  - 1984 May-Jun
TI  - Monoclonal antibody localization in subcutaneous and intracranial human glioma 
      xenografts: paired-label and imaging analysis.
PG  - 133-40
AB  - The murine antiglioma monoclonal antibody 81C6 has been shown to specifically 
      localize in U-251 MG and D-54 MG human glioma subcutaneous and intracranial 
      athymic mouse xenografts expressing the human gliomamesenchymal extracellular 
      matrix glycoprotein GMEM. In paired-label studies 81C6 reached peak levels of 
      localization in subcutaneous and intracranial xenografts in 24 to 48 hours and 
      persisted there for an additional 5 to 7 days before declining. The percent 
      localized 81C6 in U-251 MG subcutaneous xenografts was tumor size dependent 
      ranging from 2% in 200-300 mg tumors to 15% in 1 gram tumors. Subcutaneous 
      xenografts of U-251 MG were readily radioimaged from 1 up to 6 days following 
      administration of 131-I-81C6. The specificity of 81C6 localization has also been 
      demonstrated by tissue autoradiography, elution of radiolabeled 81C6 from tumor 
      xenografts, and in vivo inhibition of radiolabeled 81C6 localization by unlabeled 
      81C6. D-54 MG intracranial xenografts were permeable to 81C6 and control 
      immunoglobulin but specific localization occurred only with 81C6. Radiolabeled 
      81C6 monoclonal antibody and U-251 MG and D-54 MG human glioma xenografts provide 
      a useful operationally tumor specific monoclonal antibody tumor localization 
      model for the examination of monoclonal antibody pharmacokinetics, radioimaging, 
      radioimmunotherapy and combined modality therapy in intracranial human glioma 
      xenografts.
FAU - Bourdon, M A
AU  - Bourdon MA
FAU - Coleman, R E
AU  - Coleman RE
FAU - Blasberg, R G
AU  - Blasberg RG
FAU - Groothuis, D R
AU  - Groothuis DR
FAU - Bigner, D D
AU  - Bigner DD
LA  - eng
GR  - CA11898/CA/NCI NIH HHS/United States
GR  - CA32672/CA/NCI NIH HHS/United States
GR  - NS20023/NS/NINDS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Iodine Radioisotopes)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*analysis
MH  - Autoradiography
MH  - Brain Neoplasms/*immunology
MH  - Cell Line
MH  - Glioma/diagnostic imaging/*immunology
MH  - Humans
MH  - Immunoglobulin G/analysis
MH  - Iodine Radioisotopes
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Radionuclide Imaging
MH  - Skin Neoplasms/diagnostic imaging/*immunology
MH  - Transplantation, Heterologous
EDAT- 1984/05/01 00:00
MHDA- 1984/05/01 00:01
CRDT- 1984/05/01 00:00
PHST- 1984/05/01 00:00 [pubmed]
PHST- 1984/05/01 00:01 [medline]
PHST- 1984/05/01 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1984 May-Jun;4(3):133-40.