PMID- 6955527
OWN - NLM
STAT- MEDLINE
DCOM- 19821021
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 43
IP  - 1
DP  - 1982 Jul
TI  - Envelope gene sequences which encode the gp52 protein of spleen focus-forming 
      virus are required for the induction of erythroid cell proliferation.
PG  - 223-33
AB  - A series of insertion-deletion mutants was constructed in a molecularly cloned 
      DNA copy of the Friend strain of spleen focus-forming virus (SFFV). The mutants 
      were produced by inserting a synthetic oligonucleotide linker containing the 
      recognition sequence of SalI endonuclease into several different locations of the 
      SFFV DNA. Three classes of mutants were isolated: insertion-deletion mutants in 
      the 5' half of the SFFV genome, in the long terminal repeat of the SFFV genome, 
      and in the env gene of the SFFV genome. The env gene mutant has a deletion of 
      sequences shared in common between the env gene of SFFV and the env genes of mink 
      cell focus-inducing murine leukemia viruses. From analyses of the biological 
      activity of the various mutants and a biologically active subgenomic SFFV DNA 
      fragment described herein, we can deduce that the coding sequence encompassing 
      the env gene of SFFV is required for the biological activity. This region, 
      required for the pathogenic phenotype, cannot be larger than 1.5 kilobase pairs, 
      a size only slightly more than that sufficient to encode the nonglycosylated 
      precursor of the gp52 env gene product.
FAU - Linemeyer, D L
AU  - Linemeyer DL
FAU - Menke, J G
AU  - Menke JG
FAU - Ruscetti, S K
AU  - Ruscetti SK
FAU - Evans, L H
AU  - Evans LH
FAU - Scolnick, E M
AU  - Scolnick EM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Viral/analysis
MH  - Erythroblasts/pathology
MH  - *Genes, Viral
MH  - Leukemia, Erythroblastic, Acute/*etiology
MH  - Mutation
MH  - Oligodeoxyribonucleotides/analysis
MH  - Retroviridae/*genetics
MH  - Viral Envelope Proteins
MH  - Viral Proteins/*genetics
PMC - PMC256113
EDAT- 1982/07/01 00:00
MHDA- 1982/07/01 00:01
PMCR- 1982/07/01
CRDT- 1982/07/01 00:00
PHST- 1982/07/01 00:00 [pubmed]
PHST- 1982/07/01 00:01 [medline]
PHST- 1982/07/01 00:00 [entrez]
PHST- 1982/07/01 00:00 [pmc-release]
AID - 10.1128/JVI.43.1.223-233.1982 [doi]
PST - ppublish
SO  - J Virol. 1982 Jul;43(1):223-33. doi: 10.1128/JVI.43.1.223-233.1982.