PMID- 699044
OWN - NLM
STAT- MEDLINE
DCOM- 19781220
LR  - 20220408
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 15
IP  - 1
DP  - 1978 Sep
TI  - One molecule of diphtheria toxin fragment A introduced into a cell can kill the 
      cell.
PG  - 245-50
AB  - Erythrocyte ghosts containing a known number of molecules of purified fragment A 
      of diphtheria toxin with a constant amount of FITC-BSA as a fluorescence marker 
      were prepared by dialyzing a mixture of erythrocytes and these substances against 
      hypotonic solution. These substances were then introduced into diphtheria 
      toxin-resistant mouse L cells by virus-mediated cell fusion of the cells with the 
      ghosts, and mononuclear recipients that has fused with only one erythrocyte ghost 
      were separated in a flourescence-activated cell sorter (FACS) on the basis of 
      their cell size and fluorescence intensity. After separation, the viability of 
      cells containing known numbers of fragment A was examined by measuring 
      colony-forming ability. The results demonstrated that a single molecule of 
      fragment A was sufficient to kill a cell. This fact was confirmed by introduction 
      into cells of fragment A from an immunologically related mutant toxin, CRM 176 
      (fragment A176); this has a completely functional fragment B region, but in cell 
      extracts, the enzymic activity of its fragment A is about 10 fold less than that 
      of wild toxin. The cytotoxicity of CRM 176 is about two hundredths of that of the 
      wild-type (Uchida, Pappenheimer and Greany, 1973). As expected, about 100-200 
      fold excess of fragment A-176 was needed to kill the cells.
FAU - Yamaizumi, M
AU  - Yamaizumi M
FAU - Mekada, E
AU  - Mekada E
FAU - Uchida, T
AU  - Uchida T
FAU - Okada, Y
AU  - Okada Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Diphtheria Toxin)
RN  - 0 (Peptide Elongation Factors)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Cell Survival/*drug effects
MH  - Diphtheria Toxin/administration & dosage/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Erythrocyte Membrane
MH  - L Cells
MH  - Peptide Elongation Factors
MH  - Peptide Fragments/toxicity
EDAT- 1978/09/01 00:00
MHDA- 1978/09/01 00:01
CRDT- 1978/09/01 00:00
PHST- 1978/09/01 00:00 [pubmed]
PHST- 1978/09/01 00:01 [medline]
PHST- 1978/09/01 00:00 [entrez]
AID - 0092-8674(78)90099-5 [pii]
AID - 10.1016/0092-8674(78)90099-5 [doi]
PST - ppublish
SO  - Cell. 1978 Sep;15(1):245-50. doi: 10.1016/0092-8674(78)90099-5.