PMID- 7474079
OWN - NLM
STAT- MEDLINE
DCOM- 19951201
LR  - 20211008
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 69
IP  - 11
DP  - 1995 Nov
TI  - Cytomegalovirus infection induces high levels of cyclins, phosphorylated Rb, and 
      p53, leading to cell cycle arrest.
PG  - 6697-704
AB  - Human cytomegalovirus (HCMV) infection stimulates cellular DNA synthesis and 
      causes chromosomal damage. Because such events likely affect cellular 
      proliferation, we investigated the impact of HCMV infection on key components of 
      the cell cycle. Early after infection, HCMV induced elevated levels of cyclin E, 
      cyclin E-associated kinase activity, and two tumor suppressor proteins, p53 and 
      the retinoblastoma gene product (Rb). The steady-state concentration of Rb 
      continued to rise throughout the infection, with most of the protein remaining in 
      the highly phosphorylated form. At early times, HCMV infection also induced 
      cyclin B accumulation, which was associated with a significant increase in 
      mitosis-promoting factor activity as the infection progresses. In contrast, the 
      levels of cyclin A and cyclin A-associated kinase activity increased only at late 
      times in the infection, and the kinetics were delayed relative to those for 
      cyclins E and B. Analysis of the cellular DNA content in the infected cells by 
      flow cytometry showed a progressive shift of the cells from the G1 to the S and 
      G2/M phases of the cell cycle, leading to an accumulation of aneuploid cells at 
      late times. We propose that these HCMV-mediated perturbations result in cell 
      cycle arrest in G2/M.
FAU - Jault, F M
AU  - Jault FM
AD  - Department of Biology, University of California, San Diego, La Jolla 92093-0357, 
      USA.
FAU - Jault, J M
AU  - Jault JM
FAU - Ruchti, F
AU  - Ruchti F
FAU - Fortunato, E A
AU  - Fortunato EA
FAU - Clark, C
AU  - Clark C
FAU - Corbeil, J
AU  - Corbeil J
FAU - Richman, D D
AU  - Richman DD
FAU - Spector, D H
AU  - Spector DH
LA  - eng
GR  - AI27670/AI/NIAID NIH HHS/United States
GR  - AI36214/AI/NIAID NIH HHS/United States
GR  - CA34729/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Cyclins)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - *Cell Cycle
MH  - Cell Division
MH  - *Cell Transformation, Viral
MH  - Cells, Cultured
MH  - Cyclins/*biosynthesis
MH  - Cytomegalovirus/genetics/*physiology
MH  - DNA Replication
MH  - Fibroblasts/cytology
MH  - Humans
MH  - Kinetics
MH  - Male
MH  - Phosphorylation
MH  - Retinoblastoma Protein/*metabolism
MH  - Skin/cytology
MH  - Time Factors
MH  - Tumor Suppressor Protein p53/*metabolism
PMC - PMC189579
EDAT- 1995/11/01 00:00
MHDA- 1995/11/01 00:01
PMCR- 1995/11/01
CRDT- 1995/11/01 00:00
PHST- 1995/11/01 00:00 [pubmed]
PHST- 1995/11/01 00:01 [medline]
PHST- 1995/11/01 00:00 [entrez]
PHST- 1995/11/01 00:00 [pmc-release]
AID - 10.1128/JVI.69.11.6697-6704.1995 [doi]
PST - ppublish
SO  - J Virol. 1995 Nov;69(11):6697-704. doi: 10.1128/JVI.69.11.6697-6704.1995.