PMID- 7481799
OWN - NLM
STAT- MEDLINE
DCOM- 19951213
LR  - 20190618
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 270
IP  - 5238
DP  - 1995 Nov 10
TI  - Tertiary and quaternary structural changes in Gi alpha 1 induced by GTP 
      hydrolysis.
PG  - 954-60
AB  - Crystallographic analysis of 2.2 angstrom resolution shows that guanosine 
      triphosphate (GTP) hydrolysis triggers conformational changes in the 
      heterotrimeric G-protein alpha subunit, Gi alpha 1. The switch II and switch III 
      segments become disordered, and linker II connecting the Ras and alpha helical 
      domains moves, thus altering the structures of potential effector and beta gamma 
      binding regions. Contacts between the alpha-helical and Ras domains are weakened, 
      possibly facilitating the release of guanosine diphosphate (GDP). The amino and 
      carboxyl termini, which contain receptor and beta gamma binding determinants, are 
      disordered in the complex with GTP, but are organized into a compact microdomain 
      on GDP hydrolysis. The amino terminus also forms extensive quaternary contacts 
      with neighboring alpha subunits in the lattice, suggesting that multimers of 
      alpha subunits or heterotrimers may play a role in signal transduction.
FAU - Mixon, M B
AU  - Mixon MB
AD  - Department of Biochemistry, University of Texas Southwestern Medical Center, 
      Dallas 75235-9050, USA.
FAU - Lee, E
AU  - Lee E
FAU - Coleman, D E
AU  - Coleman DE
FAU - Berghuis, A M
AU  - Berghuis AM
FAU - Gilman, A G
AU  - Gilman AG
FAU - Sprang, S R
AU  - Sprang SR
LA  - eng
GR  - DK 46371/DK/NIDDK NIH HHS/United States
GR  - GM34497/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - I38ZP9992A (Magnesium)
SB  - IM
CIN - Science. 1995 Nov 10;270(5238):933-4. PMID: 7481796
MH  - Binding Sites
MH  - Crystallography, X-Ray
MH  - GTP-Binding Proteins/*chemistry/metabolism
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/metabolism
MH  - Guanosine Diphosphate/*metabolism
MH  - Guanosine Triphosphate/*metabolism
MH  - Hydrogen Bonding
MH  - Hydrolysis
MH  - Magnesium/metabolism
MH  - Models, Molecular
MH  - *Protein Conformation
MH  - Protein Structure, Secondary
MH  - *Protein Structure, Tertiary
EDAT- 1995/11/10 00:00
MHDA- 1995/11/10 00:01
CRDT- 1995/11/10 00:00
PHST- 1995/11/10 00:00 [pubmed]
PHST- 1995/11/10 00:01 [medline]
PHST- 1995/11/10 00:00 [entrez]
AID - 10.1126/science.270.5238.954 [doi]
PST - ppublish
SO  - Science. 1995 Nov 10;270(5238):954-60. doi: 10.1126/science.270.5238.954.