PMID- 7533852
OWN - NLM
STAT- MEDLINE
DCOM- 19950410
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 69
IP  - 4
DP  - 1995 Apr
TI  - Inhibition of vesicular stomatitis virus infection by nitric oxide.
PG  - 2208-13
AB  - Inhibitory effects of nitric oxide (NO) on vesicular stomatitis virus (VSV) 
      infection were investigated by using a VSV-susceptible mouse neuroblastoma cell 
      line, NB41A3. Productive VSV infection of NB41A3 cells was significantly 
      inhibited by an organic NO donor, S-nitro-N-acetylpenicillamine (SNAP), while the 
      control compound N-acetylpenicillamine (NAP) had no effect. Survival rate of 
      VSV-infected cells was greatly increased by the treatment with SNAP, while the 
      NAP treatment did not have any effect. Adding SNAP 30 min prior to infection 
      resulted in complete inhibition of viral production when a low multiplicity of 
      infection (MOI) was used. Substantial inhibition of viral production was also 
      obtained with treating cells 6 h earlier before infection with a higher MOI. 
      Activating the neuronal NO synthase by treating cells with N-methyl-D-aspartate 
      (NMDA) led to significant inhibition of viral production by cells infected at the 
      three doses of virus tested (MOIs of 0.1, 1, and 5). The inhibitory effect of 
      NMDA on viral infection was totally blocked by the NO synthase inhibitor 
      N-methyl-L-arginine. However, adding hemoglobin, a strong NO-binding protein and 
      thus an inactivator of NO activity, did not reverse the NMDA-induced inhibition 
      of viral production, suggesting that NO might exert its antiviral effects inside 
      the NO-producing cells. Collectively, these data support the anti-VSV effects of 
      NO, which might be one of the important factors of natural immunity in 
      controlling the initial stages of VSV infection in the central nervous system.
FAU - Bi, Z
AU  - Bi Z
AD  - Department of Biology, New York University, New York 10003-5181.
FAU - Reiss, C S
AU  - Reiss CS
LA  - eng
GR  - AI18083/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 79032-48-7 (S-Nitroso-N-Acetylpenicillamine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
RN  - GNN1DV99GX (Penicillamine)
SB  - IM
MH  - Amino Acid Oxidoreductases/metabolism
MH  - Animals
MH  - CHO Cells
MH  - Cricetinae
MH  - Enzyme Activation
MH  - Kinetics
MH  - Mice
MH  - N-Methylaspartate/pharmacology
MH  - Neurons/enzymology
MH  - Nitric Oxide/*physiology
MH  - Nitric Oxide Synthase
MH  - Penicillamine/analogs & derivatives/pharmacology
MH  - Rhabdoviridae Infections/*prevention & control/virology
MH  - S-Nitroso-N-Acetylpenicillamine
MH  - Tumor Cells, Cultured
MH  - Vesicular stomatitis Indiana virus/*physiology
MH  - Virus Replication
PMC - PMC188889
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
PMCR- 1995/04/01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
PHST- 1995/04/01 00:00 [pmc-release]
AID - 10.1128/JVI.69.4.2208-2213.1995 [doi]
PST - ppublish
SO  - J Virol. 1995 Apr;69(4):2208-13. doi: 10.1128/JVI.69.4.2208-2213.1995.