PMID- 7559628
OWN - NLM
STAT- MEDLINE
DCOM- 19951121
LR  - 20240109
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 270
IP  - 42
DP  - 1995 Oct 20
TI  - Activation of transcription factor NF-kappa B is suppressed by curcumin 
      (diferuloylmethane) [corrected].
PG  - 24995-5000
AB  - When activated, NF-kappa B, a ubiquitous transcription factor, binds DNA as a 
      heterodimeric complex composed of members of the Rel/NF-kappa B family of 
      polypeptides. Because of its intimate involvement in host defense against 
      disease, this transcription factor is an important target for therapeutic 
      intervention. In the present report we demonstrate that curcumin 
      (diferuloylmethane), a known anti-inflammatory and anticarcinogenic agent, is a 
      potent inhibitor of NF-kappa B activation. Treatment of human myeloid ML-1a cells 
      with tumor necrosis factor (TNF) rapidly activated NF-kappa B, which consists of 
      p50 and p65 subunits, and this activation was inhibited by curcumin. AP-1 binding 
      factors were also found to be down-modulated by curcumin, whereas the Sp1 binding 
      factor was unaffected. Besides TNF, curcumin also blocked phorbol ester- and 
      hydrogen peroxide-mediated activation of NF-kappa B. The TNF-dependent 
      phosphorylation and degradation of I kappa B alpha was not observed in 
      curcumin-treated cells; the translocation of p65 subunit to the nucleus was 
      inhibited at the same time. The mechanism of action of curcumin was found to be 
      different from that of protein tyrosine phosphatase inhibitors. Our results 
      indicate that curcumin inhibits NF-kappa B activation pathway at a step before I 
      kappa B alpha phosphorylation but after the convergence of various stimuli.
FAU - Singh, S
AU  - Singh S
AD  - Department of Molecular Oncology, University of Texas M.D. Anderson Cancer 
      Center, Houston 77030, USA.
FAU - Aggarwal, B B
AU  - Aggarwal BB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 9007-49-2 (DNA)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
EIN - J Biol Chem 1995 Dec 15;270(50):30235
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
MH  - Antineoplastic Agents/*pharmacology
MH  - Base Sequence
MH  - Curcumin/*pharmacology
MH  - DNA/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Humans
MH  - *I-kappa B Proteins
MH  - Molecular Sequence Data
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/*metabolism
MH  - Phosphorylation
MH  - Reactive Oxygen Species
MH  - Transcription Factor AP-1/metabolism
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1995/10/20 00:00
MHDA- 1995/10/20 00:01
CRDT- 1995/10/20 00:00
PHST- 1995/10/20 00:00 [pubmed]
PHST- 1995/10/20 00:01 [medline]
PHST- 1995/10/20 00:00 [entrez]
AID - S0021-9258(18)87080-6 [pii]
AID - 10.1074/jbc.270.42.24995 [doi]
PST - ppublish
SO  - J Biol Chem. 1995 Oct 20;270(42):24995-5000. doi: 10.1074/jbc.270.42.24995.