PMID- 7585629 OWN - NLM STAT- MEDLINE DCOM- 19951214 LR - 20220408 IS - 0008-5472 (Print) IS - 0008-5472 (Linking) VI - 55 IP - 23 DP - 1995 Dec 1 TI - Frequent expression of a mutant epidermal growth factor receptor in multiple human tumors. PG - 5536-9 AB - The epidermal growth factor receptor has received much interest as a target for various antineoplastic agents, but a complication is that many normal tissues also express this receptor. We have previously identified in human glial tumors an 801-bp in-frame deletion within the epidermal growth factor receptor gene that created a novel epitope at the junction. By using Western blot assays with a mutant-specific antibody as a rapid and sensitive means for detecting this alteration in primary human tumors, it was found that 57% (26 of 46) of high-grade and 86% (6 of 7) of low-grade glial tumors, but not normal brain, express this protein. This altered receptor was also present in 66% (4 of 6) of pediatric gliomas and 86% (6 of 7) of medulloblastomas, 78% (21 of 27) of breast carcinomas, and 73% (24 of 32) of ovarian carcinomas. The fact that this receptor is frequently found in tumors but not in normal tissue makes it an attractive candidate for various antitumor strategies. FAU - Moscatello, D K AU - Moscatello DK AD - Department of Microbiology and Immunology, Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA. FAU - Holgado-Madruga, M AU - Holgado-Madruga M FAU - Godwin, A K AU - Godwin AK FAU - Ramirez, G AU - Ramirez G FAU - Gunn, G AU - Gunn G FAU - Zoltick, P W AU - Zoltick PW FAU - Biegel, J A AU - Biegel JA FAU - Hayes, R L AU - Hayes RL FAU - Wong, A J AU - Wong AJ LA - eng GR - CA-51093/CA/NCI NIH HHS/United States GR - NS-31102/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Cancer Res JT - Cancer research JID - 2984705R RN - 0 (Antibodies, Monoclonal) RN - 0 (Neoplasm Proteins) RN - EC 2.7.10.1 (ErbB Receptors) SB - IM MH - Antibodies, Monoclonal MH - Base Sequence MH - Blotting, Western MH - Brain Neoplasms/chemistry MH - Breast Neoplasms/chemistry MH - ErbB Receptors/analysis/*genetics MH - Female MH - Humans MH - Molecular Sequence Data MH - Neoplasm Proteins/analysis/*genetics MH - Neoplasms/*chemistry MH - Ovarian Neoplasms/chemistry MH - Tumor Cells, Cultured EDAT- 1995/12/01 00:00 MHDA- 1995/12/01 00:01 CRDT- 1995/12/01 00:00 PHST- 1995/12/01 00:00 [pubmed] PHST- 1995/12/01 00:01 [medline] PHST- 1995/12/01 00:00 [entrez] PST - ppublish SO - Cancer Res. 1995 Dec 1;55(23):5536-9.