PMID- 7717456
OWN - NLM
STAT- MEDLINE
DCOM- 19950512
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 146
IP  - 4
DP  - 1995 Apr
TI  - Improved engraftment of human spleen cells in NOD/LtSz-scid/scid mice as compared 
      with C.B-17-scid/scid mice.
PG  - 888-902
AB  - T and B lymphocyte-deficient mice homozygous for the severe combined 
      immunodeficiency (SCID) mutation can be immunologically engrafted with human 
      lymphocytes. However, low levels of human peripheral blood mononuclear cell 
      engraftment are commonly observed, impeding full use of this model. We now 
      demonstrate that strain background in mice homozygous for the scid mutation is a 
      strong determinant of levels of human lymphocyte engraftment. NOD/LtSz-scid/scid 
      mice support higher levels of engraftment of both human spleen and peripheral 
      blood mononuclear cells than do C.B-17-scid/scid mice. We observed, using human 
      spleen cell injected scid mice, 1), high levels of engraftment of the host 
      peripheral lymphoid tissues with human CD45+ (leukocytes), CD3+ (T cells), CD4+ 
      (helper/inducer), and CD8+ (suppressor/cytotoxic) lymphoid cells for up to 24 
      weeks in NOD/LtSz-scid/scid mice; 2), migration of high numbers of human 
      lymphocytes to peripheral lymphoid and nonlymphoid organs in NOD/LtSz-scid/scid, 
      but not in C.B-17-scid/scid mice; 3), higher levels of serum immunoglobulin of 
      human origin in NOD/LtSz-scid/scid mice than in C.B-17-scid/scid mice; 4), 
      histological lesions characteristic of human anti-mouse xenoreactivity in 
      NOD/LtSz-scid/scid mice; and 5), human origin antibodies against filarial 
      antigens after engraftment with native human spleen cells. The use of 
      NOD/LtSz-scid/scid mice as recipients to achieve significantly enhanced human 
      lymphopoietic cell engraftment will now enable human immunity to be more easily 
      studied in animal models.
FAU - Greiner, D L
AU  - Greiner DL
AD  - Department of Medicine, University of Massachusetts, Worcester, USA.
FAU - Shultz, L D
AU  - Shultz LD
FAU - Yates, J
AU  - Yates J
FAU - Appel, M C
AU  - Appel MC
FAU - Perdrizet, G
AU  - Perdrizet G
FAU - Hesselton, R M
AU  - Hesselton RM
FAU - Schweitzer, I
AU  - Schweitzer I
FAU - Beamer, W G
AU  - Beamer WG
FAU - Shultz, K L
AU  - Shultz KL
FAU - Pelsue, S C
AU  - Pelsue SC
AU  - et al.
LA  - eng
GR  - AI 27773/AI/NIAID NIH HHS/United States
GR  - AI 30046/AI/NIAID NIH HHS/United States
GR  - AI 30389/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, CD)
RN  - 0 (Gonadal Steroid Hormones)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/immunology
MH  - Antigens, CD/immunology
MH  - Blotting, Western
MH  - Brugia malayi/immunology
MH  - *Cell Transplantation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Gonadal Steroid Hormones/physiology
MH  - Graft Survival/*immunology
MH  - Humans
MH  - Leukocytes, Mononuclear/transplantation
MH  - Male
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Spleen/*cytology
MH  - Transplantation, Heterologous/*immunology
PMC - PMC1869266
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
PMCR- 1995/10/01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
PHST- 1995/10/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1995 Apr;146(4):888-902.