PMID- 7794812
OWN - NLM
STAT- MEDLINE
DCOM- 19950803
LR  - 20131121
IS  - 1044-9523 (Print)
IS  - 1044-9523 (Linking)
VI  - 6
IP  - 4
DP  - 1995 Apr
TI  - Human neuroblastoma I-type cells are malignant neural crest stem cells.
PG  - 449-56
AB  - Human neuroblastoma I-type cells isolated from cell lines in vitro are 
      morphologically intermediate between neuroblastic (N) cells, with properties of 
      embryonic sympathoblasts, and substrate-adherent (S) cells having properties of 
      embryonic Schwann/glial/melanocytic cells of the neural crest. I cells have 
      biochemical features of both N and S cells. We propose that the I-type cell 
      represents a malignant neural crest stem cell. The strongest evidence in support 
      of this hypothesis is that: (a) I cells can generate progeny that have neuronal 
      properties, i.e., are committed neuroblasts, or properties of nonneuronal, 
      embryonic neural crest-derived cells; and (b) I-type cells can generate 
      multipotent I-type progeny, indicating their capacity for self-renewal, a feature 
      of stem cells. We report here that I-type cells, derived from four different 
      human neuroblastoma cell lines and experimentally induced to differentiate, give 
      rise to cells with distinct N or S cell phenotypes, indicative of I cell 
      multipotentiality. Experiments with a large panel of I-type subclones, isolated 
      from clonal I-type BE(2)-C cells and exposed to retinoic acid to induce neuronal 
      differentiation or 5-bromo-2'-deoxyuridine to obtain S-type cells, demonstrated 
      that differentiation occurs via induction and selection and not by selection of 
      spontaneously arising variants. The differentiation phenotype was stable. We 
      conclude that human neuroblastoma I-type cells are multipotent embryonic 
      precursor cells of the peripheral nervous system, capable of either neuronal or 
      nonneuronal neural crest cell differentiation.
FAU - Ross, R A
AU  - Ross RA
AD  - Department of Biological Sciences, Fordham University, Bronx, New York 10458, 
      USA.
FAU - Spengler, B A
AU  - Spengler BA
FAU - Domenech, C
AU  - Domenech C
FAU - Porubcin, M
AU  - Porubcin M
FAU - Rettig, W J
AU  - Rettig WJ
FAU - Biedler, J L
AU  - Biedler JL
LA  - eng
GR  - CA08748/CA/NCI NIH HHS/United States
GR  - CA41520/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell Growth Differ
JT  - Cell growth & differentiation : the molecular biology journal of the American 
      Association for Cancer Research
JID - 9100024
RN  - 5688UTC01R (Tretinoin)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Bromodeoxyuridine/pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cell Transformation, Neoplastic
MH  - Clone Cells
MH  - Humans
MH  - Neural Crest/*pathology
MH  - Neuroblastoma/*pathology
MH  - Stem Cells/drug effects/*pathology
MH  - Tretinoin/pharmacology
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
PST - ppublish
SO  - Cell Growth Differ. 1995 Apr;6(4):449-56.