PMID- 7814646
OWN - NLM
STAT- MEDLINE
DCOM- 19950209
LR  - 20220318
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 95
IP  - 1
DP  - 1995 Jan
TI  - A distinct array of proinflammatory cytokines is expressed in human colon 
      epithelial cells in response to bacterial invasion.
PG  - 55-65
AB  - Pathogenic bacteria that penetrate the intestinal epithelial barrier stimulate an 
      inflammatory response in the adjacent intestinal mucosa. The present studies 
      asked whether colon epithelial cells can provide signals that are important for 
      the initiation and amplification of an acute mucosal inflammatory response. 
      Infection of monolayers of human colon epithelial cell lines (T84, HT29, Caco-2) 
      with invasive strains of bacteria (Salmonella dublin, Shigella dysenteriae, 
      Yersinia enterocolitica, Listeria monocytogenes, enteroinvasive Escherichia coli) 
      resulted in the coordinate expression and upregulation of a specific array of 
      four proinflammatory cytokines, IL-8, monocyte chemotactic protein-1, GM-CSF, and 
      TNF alpha, as assessed by mRNA levels and cytokine secretion. Expression of the 
      same cytokines was upregulated after TNF alpha or IL-1 stimulation of these 
      cells. In contrast, cytokine gene expression was not altered after infection of 
      colon epithelial cells with noninvasive bacteria or the noninvasive protozoan 
      parasite, G. lamblia. Notably, none of the cell lines expressed mRNA for IL-2, 
      IL-4, IL-5, IL-6, IL-12p40, IFN-gamma, or significant levels of IL-1 or IL-10 in 
      response to the identical stimuli. The coordinate expression of IL-8, MCP-1, 
      GM-CSF and TNF alpha appears to be a general property of human colon epithelial 
      cells since an identical array of cytokines, as well as IL-6, also was expressed 
      by freshly isolated human colon epithelial cells. Since the cytokines expressed 
      in response to bacterial invasion or other proinflammatory agonists have a well 
      documented role in chemotaxis and activation of inflammatory cells, colon 
      epithelial cells appear to be programmed to provide a set of signals for the 
      activation of the mucosal inflammatory response in the earliest phases after 
      microbial invasion.
FAU - Jung, H C
AU  - Jung HC
AD  - Department of Medicine, University of California, San Diego, La Jolla 92093-0623.
FAU - Eckmann, L
AU  - Eckmann L
FAU - Yang, S K
AU  - Yang SK
FAU - Panja, A
AU  - Panja A
FAU - Fierer, J
AU  - Fierer J
FAU - Morzycka-Wroblewska, E
AU  - Morzycka-Wroblewska E
FAU - Kagnoff, M F
AU  - Kagnoff MF
LA  - eng
GR  - DK-35108/DK/NIDDK NIH HHS/United States
GR  - DK-40582/DK/NIDDK NIH HHS/United States
GR  - DK-47739/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
CIN - J Clin Invest. 2001 Jan;107(1):27-30. PMID: 11134175
MH  - Animals
MH  - Bacterial Infections/*immunology
MH  - Base Sequence
MH  - Cell Line
MH  - Chemokine CCL2
MH  - Chemotactic Factors/biosynthesis
MH  - Colonic Diseases/*immunology
MH  - Cytokines/*biosynthesis/genetics
MH  - Epithelial Cells
MH  - Epithelium/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Giardia lamblia
MH  - Giardiasis/metabolism
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/biosynthesis
MH  - Humans
MH  - Inflammation/*metabolism
MH  - Interleukin-8/biosynthesis
MH  - Molecular Sequence Data
MH  - RNA, Messenger/analysis
MH  - Tumor Necrosis Factor-alpha/biosynthesis/pharmacology
PMC - PMC295369
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
PMCR- 1995/01/01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PHST- 1995/01/01 00:00 [pmc-release]
AID - 10.1172/JCI117676 [doi]
PST - ppublish
SO  - J Clin Invest. 1995 Jan;95(1):55-65. doi: 10.1172/JCI117676.