PMID- 7821921
OWN - NLM
STAT- MEDLINE
DCOM- 19950214
LR  - 20220408
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 26
IP  - 1
DP  - 1995 Jan
TI  - Expression of vascular permeability factor (vascular endothelial growth factor) 
      and its receptors in breast cancer.
PG  - 86-91
AB  - Solid tumors must induce a vascular stroma to grow beyond a minimal size, and the 
      intensity of the angiogenic response has been correlated with prognosis in breast 
      cancer patients. Vascular permeability factor (VPF), also known as vascular 
      endothelial growth factor (VEGF), is a secreted protein that has been implicated 
      in tumor-associated angiogenesis. Vascular permeability factor directly 
      stimulates endothelial cell growth and also increases microvascular permeability, 
      leading to the extravasation of plasma proteins, which alter the extracellular 
      matrix in a manner that promotes angiogenesis. To determine whether VPF has a 
      role in breast cancer, we used in situ hybridization to study VPF mRNA expression 
      in normal breast tissue (13 specimens), comedo-type ductal carcinoma in situ 
      (DCIS) (four specimens), infiltrating ductal carcinoma (12 specimens), 
      infiltrating lobular carcinoma (two specimens), metastatic ductal carcinoma 
      (three specimens) and metastatic lobular carcinoma (one specimen). Vascular 
      permeability factor mRNA was expressed at a low level by normal duct epithelium 
      but was expressed at high levels in tumor cells in all cases of comedo-type DCIS, 
      infiltrating ductal carcinoma, and metastatic ductal carcinoma. In contrast, VPF 
      mRNA was not expressed at high levels in infiltrating lobular carcinoma. We also 
      used in situ hybridization to study the expression of two recently described 
      endothelial cell surface VPF receptors, flt-1 and kdr. Vascular permeability 
      factor receptor mRNA was strongly expressed in endothelial cells of small vessels 
      adjacent to malignant tumor cells in DCIS, infiltrating ductal carcinoma, and 
      metastatic ductal carcinoma. In contrast, no definite labeling for receptor mRNA 
      was detected in infiltrating lobular carcinoma or nonmalignant breast tissue. The 
      intense expression of VPF mRNA by breast carcinoma cells and of VPF receptor mRNA 
      by endothelial cells of adjacent small blood vessels provides strong evidence 
      linking VPF expression to the angiogenesis associated with comedo-type DCIS, 
      infiltrating ductal, and metastatic ductal breast carcinoma.
FAU - Brown, L F
AU  - Brown LF
AD  - Department of Pathology, Beth Israel Hospital, Boston, MA 02215.
FAU - Berse, B
AU  - Berse B
FAU - Jackman, R W
AU  - Jackman RW
FAU - Tognazzi, K
AU  - Tognazzi K
FAU - Guidi, A J
AU  - Guidi AJ
FAU - Dvorak, H F
AU  - Dvorak HF
FAU - Senger, D R
AU  - Senger DR
FAU - Connolly, J L
AU  - Connolly JL
FAU - Schnitt, S J
AU  - Schnitt SJ
LA  - eng
GR  - CA43967/CA/NCI NIH HHS/United States
GR  - CA50453/CA/NCI NIH HHS/United States
GR  - CA58845/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptors, Vascular Endothelial Growth Factor)
SB  - IM
MH  - Breast Neoplasms/*metabolism
MH  - Carcinoma/*metabolism
MH  - Carcinoma in Situ/*metabolism
MH  - Carcinoma, Ductal, Breast/*metabolism
MH  - Endothelial Growth Factors/genetics/*metabolism
MH  - Female
MH  - Humans
MH  - In Situ Hybridization
MH  - Lymphokines/genetics/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptor Protein-Tyrosine Kinases/genetics/*metabolism
MH  - Receptors, Growth Factor/genetics/*metabolism
MH  - Receptors, Vascular Endothelial Growth Factor
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 0046-8177(95)90119-1 [pii]
AID - 10.1016/0046-8177(95)90119-1 [doi]
PST - ppublish
SO  - Hum Pathol. 1995 Jan;26(1):86-91. doi: 10.1016/0046-8177(95)90119-1.