PMID- 7854453
OWN - NLM
STAT- MEDLINE
DCOM- 19950316
LR  - 20220318
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 373
IP  - 6516
DP  - 1995 Feb 23
TI  - Placental defect and embryonic lethality in mice lacking hepatocyte growth 
      factor/scatter factor.
PG  - 702-5
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) functions as a mitogen, motogen 
      and morphogen for a variety of cultured cells. The genes for HGF/SF and its 
      receptor (the c-met proto-oncogene product) are expressed in many tissues during 
      the embryonic periods and in the adult. HGF/SF is thought to mediate a signal 
      exchange between the mesenchyme and epithelia during mouse development. To 
      examine the physiological role of HGF/SF, we generated mutant mice with a 
      targeted disruption of the HGF/SF gene. Here we report that homozygous mutant 
      embryos have severely impaired placentas with markedly reduced numbers of 
      labyrinthine trophoblast cells, and die before birth. The growth of trophoblast 
      cells was stimulated by HGF/SF in vitro, and the HGF/SF activity was released by 
      allantois in primary culture of normal but not mutant embryos. These findings 
      suggest that HGF/SF is an essential mediator of allantoic 
      mesenchyme-trophoblastic epithelia interaction required for placental 
      organogenesis.
FAU - Uehara, Y
AU  - Uehara Y
AD  - Institute for Liver Research, Kansai Medical University, Moriguchi, Osaka, Japan.
FAU - Minowa, O
AU  - Minowa O
FAU - Mori, C
AU  - Mori C
FAU - Shiota, K
AU  - Shiota K
FAU - Kuno, J
AU  - Kuno J
FAU - Noda, T
AU  - Noda T
FAU - Kitamura, N
AU  - Kitamura N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (DNA Primers)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
GS  - HGF
GS  - SF
MH  - Allantois/metabolism/pathology
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA Primers
MH  - Embryonic and Fetal Development/genetics/*physiology
MH  - Fetal Death
MH  - Hepatocyte Growth Factor/deficiency/genetics/*physiology
MH  - Homozygote
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Mutagenesis
MH  - Placenta/abnormalities/*embryology
MH  - Stem Cells
MH  - Trophoblasts/pathology
EDAT- 1995/02/23 00:00
MHDA- 1995/02/23 00:01
CRDT- 1995/02/23 00:00
PHST- 1995/02/23 00:00 [pubmed]
PHST- 1995/02/23 00:01 [medline]
PHST- 1995/02/23 00:00 [entrez]
AID - 10.1038/373702a0 [doi]
PST - ppublish
SO  - Nature. 1995 Feb 23;373(6516):702-5. doi: 10.1038/373702a0.