PMID- 7857643
OWN - NLM
STAT- MEDLINE
DCOM- 19950320
LR  - 20240104
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 14
IP  - 2
DP  - 1995 Feb
TI  - Targeted expression of tetanus toxin light chain in Drosophila specifically 
      eliminates synaptic transmission and causes behavioral defects.
PG  - 341-51
AB  - Tetanus toxin cleaves the synaptic vesicle protein synaptobrevin, and the ensuing 
      loss of neurotransmitter exocytosis has implicated synaptobrevin in this process. 
      To further the study of synaptic function in a genetically tractable organism and 
      to generate a tool to disable neuronal communication for behavioural studies, we 
      have expressed a gene encoding tetanus toxin light chain in Drosophila. Toxin 
      expression in embryonic neurons removes detectable synaptobrevin and eliminates 
      evoked, but not spontaneous, synaptic vesicle release. No other developmental or 
      morphological defects are detected. Correspondingly, only synaptobrevin (n-syb), 
      but not the ubiquitously expressed syb protein, is cleaved by tetanus toxin in 
      vitro. Targeted expression of toxin can produce specific behavioral defects; in 
      one case, the olfactory escape response is reduced.
FAU - Sweeney, S T
AU  - Sweeney ST
AD  - Department of Genetics, University of Cambridge, England.
FAU - Broadie, K
AU  - Broadie K
FAU - Keane, J
AU  - Keane J
FAU - Niemann, H
AU  - Niemann H
FAU - O'Kane, C J
AU  - O'Kane CJ
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Hormones)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (R-SNARE Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Syb protein, Drosophila)
RN  - 0 (Tetanus Toxin)
RN  - 0 (Vesicular Transport Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Conserved Sequence
MH  - Drosophila/*physiology
MH  - *Drosophila Proteins
MH  - Embryo, Nonmammalian/physiology
MH  - *Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - Insect Hormones/analysis/biosynthesis
MH  - Macromolecular Substances
MH  - Membrane Proteins/analysis/chemistry/*metabolism
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/analysis/chemistry/*metabolism
MH  - Neuromuscular Junction/physiology
MH  - Neuropeptides/analysis/biosynthesis
MH  - R-SNARE Proteins
MH  - Rats
MH  - Recombinant Proteins/biosynthesis
MH  - Sequence Homology, Amino Acid
MH  - Synapses/*physiology
MH  - Synaptic Transmission/*physiology
MH  - Tetanus Toxin/analysis/*biosynthesis/metabolism
MH  - *Vesicular Transport Proteins
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 0896-6273(95)90290-2 [pii]
AID - 10.1016/0896-6273(95)90290-2 [doi]
PST - ppublish
SO  - Neuron. 1995 Feb;14(2):341-51. doi: 10.1016/0896-6273(95)90290-2.