PMID- 7882332
OWN - NLM
STAT- MEDLINE
DCOM- 19950412
LR  - 20061115
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 55
IP  - 6
DP  - 1995 Mar 15
TI  - Gains and losses of DNA sequences in osteosarcomas by comparative genomic 
      hybridization.
PG  - 1334-8
AB  - Our aim was to identify chromosomal regions that are likely to harbor previously 
      unknown genes with an important role in the genesis of osteosarcoma. Comparative 
      genomic hybridization was used to screen for losses and gains of DNA sequences 
      along all chromosome arms in 11 tumors. Extensive genetic aberrations, with an 
      average of 11 changes/tumor (range, 1-20), were found in 10 of the 11 specimens. 
      High level amplifications of small chromosomal regions were detected in eight 
      tumors. These involved the 12q12-q13 region (known to contain the SAS-MDM2 locus) 
      and several previously unreported amplification sites such as 17p11-p12, 3q26, 
      and Xq12. When all DNA sequence gains were evaluated, the gains at 8q and Xp were 
      most common (45%). The most common losses of DNA sequences were seen at 2q, 6q, 
      8p, and 10p (36%). In conclusion, despite the very complex pattern of genetic 
      changes in osteosarcomas, certain chromosomal regions appear to be affected more 
      often than others. Most of these regions have not previously been reported to be 
      implicated in osteosarcomas and may thus highlight locations of novel genes with 
      an important role in the development and progression of these tumors.
FAU - Tarkkanen, M
AU  - Tarkkanen M
AD  - Department of Medical Genetics, University of Helsinki, Finland.
FAU - Karhu, R
AU  - Karhu R
FAU - Kallioniemi, A
AU  - Kallioniemi A
FAU - Elomaa, I
AU  - Elomaa I
FAU - Kivioja, A H
AU  - Kivioja AH
FAU - Nevalainen, J
AU  - Nevalainen J
FAU - Bohling, T
AU  - Bohling T
FAU - Karaharju, E
AU  - Karaharju E
FAU - Hyytinen, E
AU  - Hyytinen E
FAU - Knuutila, S
AU  - Knuutila S
AU  - et al.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Gene Amplification
MH  - Humans
MH  - Nucleic Acid Hybridization
MH  - Osteosarcoma/*genetics
EDAT- 1995/03/15 00:00
MHDA- 1995/03/15 00:01
CRDT- 1995/03/15 00:00
PHST- 1995/03/15 00:00 [pubmed]
PHST- 1995/03/15 00:01 [medline]
PHST- 1995/03/15 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1995 Mar 15;55(6):1334-8.