PMID- 7891077
OWN - NLM
STAT- MEDLINE
DCOM- 19950418
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 64
IP  - 4
DP  - 1995 Apr
TI  - Cytokines regulate gelatinase A and B (matrix metalloproteinase 2 and 9) activity 
      in cultured rat astrocytes.
PG  - 1513-20
AB  - Under a tightly regulated expression mechanism, matrix metalloproteinases degrade 
      extracellular matrix proteins and are thought to play a role in injury repair and 
      tumor metastasis in peripheral tissues. Little is known about the function of 
      matrix metalloproteinases or agents that regulate their production in adult 
      brain; however, it has been shown that the activity of a calcium-dependent 
      metalloproteinase is elevated in Alzheimer's hippocampus. The goals of this study 
      were to determine whether cultured rat astrocytes produce matrix 
      metalloproteinases and to identify agents that regulate protease activity. 
      Enriched astrocyte cultures were prepared from brains of 1-day-old rat pups, and 
      experiments were performed 13 days later. Gelatinase activity in astrocyte 
      conditioned medium was determined using zymography with gelatin copolymerized 
      with acrylamide in the gel. Under basal conditions after a 24-h incubation, rat 
      astrocytes produce gelatinases of 58 and 66 kDa. On stimulation of astrocytes 
      with lipopolysaccharide, interleukin-1 alpha or -beta, or tumor necrosis 
      factor-alpha for 24 h, a dose-dependent increase in the activity of the 58- and 
      66-kDa gelatinases and the induction of a 94-kDa gelatinase occurred. All three 
      astrocyte-derived proteases showed maximal activity in the presence of millimolar 
      levels of Ca2+, their activity was inhibited in the presence of 
      1,10-phenanthroline, and their proenzymes were cleaved and activated after 
      incubation with p-aminophenylmercuric acetate. Using immunoblotting, 
      immunopositive bands at the respective molecular sizes indicated that the 58-kDa 
      gelatinase was gelatinase A (matrix metalloproteinase 2) and the 94-kDa activity 
      was gelatinase B (matrix metalloproteinase 9).(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Gottschall, P E
AU  - Gottschall PE
AD  - Department of Pharmacology and Therapeutics, University of South Florida College 
      of Medicine, Tampa 33612-4799.
FAU - Yu, X
AU  - Yu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Interleukin-1)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Gelatinases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Astrocytes/*enzymology
MH  - Cells, Cultured
MH  - Collagenases/*metabolism
MH  - Cytokines/*pharmacology
MH  - Dexamethasone/pharmacology
MH  - Extracellular Matrix/enzymology
MH  - Gelatinases/*metabolism
MH  - Immunoblotting
MH  - Interleukin-1/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Matrix Metalloproteinase 2
MH  - Matrix Metalloproteinase 9
MH  - Metalloendopeptidases/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Interleukin-1/antagonists & inhibitors
EDAT- 1995/04/01 00:00
MHDA- 1995/04/01 00:01
CRDT- 1995/04/01 00:00
PHST- 1995/04/01 00:00 [pubmed]
PHST- 1995/04/01 00:01 [medline]
PHST- 1995/04/01 00:00 [entrez]
AID - 10.1046/j.1471-4159.1995.64041513.x [doi]
PST - ppublish
SO  - J Neurochem. 1995 Apr;64(4):1513-20. doi: 10.1046/j.1471-4159.1995.64041513.x.