PMID- 7912657
OWN - NLM
STAT- MEDLINE
DCOM- 19940804
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 77
IP  - 7
DP  - 1994 Jul 1
TI  - Activation and depalmitoylation of Gs alpha.
PG  - 1063-70
AB  - [3H]palmitate attached to mutationally activated alpha s (alpha s-R201C) turns 
      over rapidly, compared with palmitate linked to normal alpha s (t1/2 
      approximately 2 min versus 90 min); although alpha s-R201C (unlike normal alpha 
      s) is predominantly found in the cytosol, [3H]palmitate is linked only to the 
      membrane-bound pool of alpha s, normal or mutant. Similarly, activation of 
      wild-type alpha s by isoproterenol, a beta-adrenoceptor agonist that also induces 
      membrane-to-cytosol translocation of alpha s, dramatically accelerates 
      depalmitoylation of alpha s. Thus, activation-induced removal of palmitate 
      provides an explanation for activation-induced shifts of alpha s to the cytosol. 
      Regulated palmitoylation cycles provide a potential general mechanism for 
      controlling reversible changes in the cellular location and activity of a 
      protein.
FAU - Wedegaertner, P B
AU  - Wedegaertner PB
AD  - Department of Pharmacology, University of California, San Francisco 94143.
FAU - Bourne, H R
AU  - Bourne HR
LA  - eng
GR  - CA-54427/CA/NCI NIH HHS/United States
GR  - GM-27800/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Adrenergic beta-Agonists)
RN  - 0 (Palmitates)
RN  - 1F7A44V6OU (Colforsin)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Adrenergic beta-Agonists/pharmacology
MH  - Animals
MH  - Cell Compartmentation
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - Colforsin/pharmacology
MH  - Enzyme Activation
MH  - GTP Phosphohydrolases/antagonists & inhibitors
MH  - GTP-Binding Proteins/*metabolism
MH  - Isoproterenol/pharmacology
MH  - Mice
MH  - Palmitates/metabolism
MH  - Protein Processing, Post-Translational
MH  - Signal Transduction
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 0092-8674(94)90445-6 [pii]
AID - 10.1016/0092-8674(94)90445-6 [doi]
PST - ppublish
SO  - Cell. 1994 Jul 1;77(7):1063-70. doi: 10.1016/0092-8674(94)90445-6.