PMID- 8015608
OWN - NLM
STAT- MEDLINE
DCOM- 19940725
LR  - 20220330
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 370
IP  - 6484
DP  - 1994 Jul 7
TI  - A matrix metalloproteinase expressed on the surface of invasive tumour cells.
PG  - 61-5
AB  - Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma 
      cells and is believed to be crucial for their invasion and metastasis, acting by 
      degrading extracellular matrix macro-molecules such as type IV collagen. An 
      inactive precursor of gelatinase A (pro-gelatinase A) is secreted and activated 
      in invasive tumour tissue as a result of proteolysis which is mediated by a 
      fraction of tumour cell membrane that is sensitive to metalloproteinase 
      inhibitors. Here we report the cloning of the complementary DNA encoding a new 
      matrix metalloproteinase with a potential transmembrane domain. Expression of the 
      gene product on the cell surface induces specific activation of pro-gelatinase A 
      in vitro and enhances cellular invasion of the reconstituted basement membrane. 
      Tumour cells of invasive lung carcinomas, which contain activated forms of 
      gelatinase A, were found to express the transcript and the gene product. The new 
      metalloproteinase may thus trigger invasion by tumour cells by activating 
      pro-gelatinase A on the tumour cell surface.
FAU - Sato, H
AU  - Sato H
AD  - Department of Molecular Virology and Oncology, School of Medicine, Kanazawa 
      University, Ishikawa, Japan.
FAU - Takino, T
AU  - Takino T
FAU - Okada, Y
AU  - Okada Y
FAU - Cao, J
AU  - Cao J
FAU - Shinagawa, A
AU  - Shinagawa A
FAU - Yamamoto, E
AU  - Yamamoto E
FAU - Seiki, M
AU  - Seiki M
LA  - eng
SI  - GENBANK/D26512
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Enzyme Precursors)
RN  - EC 3.4.24.- (Gelatinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases, Membrane-Associated)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.- (progelatinase)
SB  - IM
CIN - Nature. 1994 Jul 7;370(6484):14-5. PMID: 8015594
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cell Membrane/*enzymology
MH  - Cloning, Molecular
MH  - Enzyme Activation
MH  - Enzyme Precursors/metabolism
MH  - Extracellular Matrix/*enzymology
MH  - Gelatinases/metabolism
MH  - Humans
MH  - Lung Neoplasms
MH  - Matrix Metalloproteinases, Membrane-Associated
MH  - Metalloendopeptidases/*biosynthesis/genetics/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Neoplasm Invasiveness
MH  - Sequence Homology, Amino Acid
MH  - Tumor Cells, Cultured
EDAT- 1994/07/07 00:00
MHDA- 2000/03/23 09:00
CRDT- 1994/07/07 00:00
PHST- 1994/07/07 00:00 [pubmed]
PHST- 2000/03/23 09:00 [medline]
PHST- 1994/07/07 00:00 [entrez]
AID - 10.1038/370061a0 [doi]
PST - ppublish
SO  - Nature. 1994 Jul 7;370(6484):61-5. doi: 10.1038/370061a0.