PMID- 8036013
OWN - NLM
STAT- MEDLINE
DCOM- 19940815
LR  - 20181130
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 9
IP  - 8
DP  - 1994 Aug
TI  - Functional characterization of an EGF receptor with a truncated extracellular 
      domain expressed in glioblastomas with EGFR gene amplification.
PG  - 2313-20
AB  - The most common type of alteration of the epidermal growth factor receptor gene 
      (EGFR) in human glioblastomas results in the synthesis of an aberrant mRNA 
      lacking 801 bases that encode amino acids 6-273 of the receptor's extracellular 
      domain. To study the effects of this mutation on receptor function, we have 
      developed chinese hamster ovary cell transfectants which express the mutant EGF 
      receptor. Comparison of wild-type and mutant receptor properties in this cell 
      host indicates that the truncated receptor does not bind EGF or TGF-alpha and, 
      consequently, DNA synthesis is not stimulated in cultures of mutant transfectants 
      by either grown factor. However, levels of DNA synthesis determined for mutant 
      transfectants in serum-free media are several-fold higher than those determined 
      for corresponding cultures of wild-type transfectants. Western blot analysis with 
      anti-phosphotyrosine antibody indicates that the mutant receptor is 
      constitutively phosphorylated in CHO cells, and the same analysis applied to 
      lysates of glioblastoma biopsies reveals the altered receptor is readily 
      detectable as a phosphotyrosine protein in tumors for which there is evidence of 
      corresponding EGFR gene and transcript alterations. In total, these results 
      indicate that the aberrant EGF receptor synthesized in glioblastomas, and which 
      lacks a portion of the extracellular domain necessary for ligand binding, is an 
      activated tyrosine kinase.
FAU - Ekstrand, A J
AU  - Ekstrand AJ
AD  - Department of Neurosurgery, Emory University SOM, Atlanta, Georgia 30322.
FAU - Longo, N
AU  - Longo N
FAU - Hamid, M L
AU  - Hamid ML
FAU - Olson, J J
AU  - Olson JJ
FAU - Liu, L
AU  - Liu L
FAU - Collins, V P
AU  - Collins VP
FAU - James, C D
AU  - James CD
LA  - eng
GR  - CA55728/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Cricetinae
MH  - ErbB Receptors/chemistry/genetics/*physiology
MH  - Glioblastoma/*genetics
MH  - Humans
MH  - *Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Transfection
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1994 Aug;9(8):2313-20.