PMID- 8070411
OWN - NLM
STAT- MEDLINE
DCOM- 19940929
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 13
IP  - 16
DP  - 1994 Aug 15
TI  - Sp1-mediated transcriptional activation is repressed by Sp3.
PG  - 3843-51
AB  - Sp1, Sp3 (SPR-2) and Sp4 (SPR-1) are human sequence-specific DNA binding proteins 
      with very similar structural features. In this report, we have analyzed Sp3 in 
      direct comparison with Sp1. We have raised antibodies against both Sp1 and Sp3, 
      and show that Sp3 protein, like Sp1, is expressed in various cell lines. 
      Co-transfection experiments in different mammalian cell lines reveal that in 
      contrast to Sp1 and Sp4, Sp3 is not able to activate several Sp1 responsive 
      promoters. In addition, Sp3 also fails to activate reporter constructs in 
      Drosophila SL2 cells lacking endogenous Sp factors. Instead, we find that Sp3 
      represses Sp1-mediated activation in a linear dose-dependent manner. A mutant of 
      Sp3 lacking the DNA binding domain does not affect activation by Sp1, suggesting 
      that the inhibition is most likely due to the competition with Sp1 for their 
      common binding sites. To determine if any structurally similar domain of Sp3 is 
      able to replace partially homologous domains of Sp1, we have generated chimeric 
      proteins and tested their activation characteristics in gene transfer 
      experiments. It appears that neither the glutamine-rich domains A and B nor the D 
      domain of Sp1 can be replaced by the homologous regions of Sp3. Our results 
      suggest that Sp3 is an inhibitory member of the Sp family.
FAU - Hagen, G
AU  - Hagen G
AD  - Institut fur Molekularbiologie und Tumorforschung, Philipps-Universitat Marburg, 
      Germany.
FAU - Muller, S
AU  - Muller S
FAU - Beato, M
AU  - Beato M
FAU - Suske, G
AU  - Suske G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Cornified Envelope Proline-Rich Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SP3 protein, human)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Transcription Factors)
RN  - 148710-94-5 (Sp3 Transcription Factor)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding, Competitive
MH  - Cell Line
MH  - Cornified Envelope Proline-Rich Proteins
MH  - DNA-Binding Proteins/immunology/*metabolism
MH  - Drosophila/cytology/genetics
MH  - *Gene Expression Regulation
MH  - Genes, Reporter
MH  - Humans
MH  - Membrane Proteins
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/metabolism
MH  - Protein Binding
MH  - Proteins/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Sp1 Transcription Factor/*metabolism
MH  - Sp3 Transcription Factor
MH  - Transcription Factors/immunology/*metabolism
MH  - *Transcription, Genetic
MH  - Transfection
PMC - PMC395297
EDAT- 1994/08/15 00:00
MHDA- 1994/08/15 00:01
PMCR- 1995/08/15
CRDT- 1994/08/15 00:00
PHST- 1994/08/15 00:00 [pubmed]
PHST- 1994/08/15 00:01 [medline]
PHST- 1994/08/15 00:00 [entrez]
PHST- 1995/08/15 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1994.tb06695.x [doi]
PST - ppublish
SO  - EMBO J. 1994 Aug 15;13(16):3843-51. doi: 10.1002/j.1460-2075.1994.tb06695.x.