PMID- 8181062
OWN - NLM
STAT- MEDLINE
DCOM- 19940614
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 77
IP  - 3
DP  - 1994 May 6
TI  - Competition between chromatin and transcription complex assembly regulates gene 
      expression during early development.
PG  - 439-49
AB  - Xenopus early development is characterized by a generalized absence of 
      transcription, which resumes at the midblastula transition (MBT). We analyzed 
      this regulation using a plasmid containing the c-myc promoter that is under the 
      same developmental control when injected into fertilized eggs. We find that the 
      repression of transcription can be relieved simply by preincubating the reporter 
      plasmid with TATA binding protein (TBP). However, the repression of gene activity 
      normally occurring before the MBT soon becomes dominant over this activation 
      independent of cell cycle phases. This inactivation correlates with chromatin 
      assembly, and titration of chromatin components not only relieves repression of 
      TBP-dependent transcription but also permits the establishment of stable 
      transcription during early development. Our data suggest that the large excess of 
      histones represses gene activity during early development through a dynamic 
      competition between chromatin assembly and transcription complex assembly.
FAU - Prioleau, M N
AU  - Prioleau MN
AD  - Institut Jacques Monod, Molecular Embryology Unit, Paris, France.
FAU - Huet, J
AU  - Huet J
FAU - Sentenac, A
AU  - Sentenac A
FAU - Mechali, M
AU  - Mechali M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Chromatin)
RN  - 0 (DNA, Superhelical)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (RNA, Messenger)
RN  - 0 (TATA-Box Binding Protein)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Animals
MH  - Chromatin/*metabolism
MH  - DNA Replication/physiology
MH  - DNA, Superhelical/metabolism
MH  - DNA-Binding Proteins/isolation & purification/*metabolism
MH  - Embryo, Nonmammalian/*metabolism
MH  - G2 Phase/physiology
MH  - Gene Expression Regulation/*physiology
MH  - Microinjections
MH  - Oogenesis
MH  - Promoter Regions, Genetic/genetics
MH  - Proto-Oncogene Proteins c-myc/genetics
MH  - RNA Polymerase II/genetics
MH  - RNA, Messenger/analysis
MH  - TATA-Box Binding Protein
MH  - Transcription Factors/isolation & purification/*metabolism
MH  - Transcriptional Activation/*genetics
MH  - Xenopus/embryology
EDAT- 1994/05/06 00:00
MHDA- 1994/05/06 00:01
CRDT- 1994/05/06 00:00
PHST- 1994/05/06 00:00 [pubmed]
PHST- 1994/05/06 00:01 [medline]
PHST- 1994/05/06 00:00 [entrez]
AID - 0092-8674(94)90158-9 [pii]
AID - 10.1016/0092-8674(94)90158-9 [doi]
PST - ppublish
SO  - Cell. 1994 May 6;77(3):439-49. doi: 10.1016/0092-8674(94)90158-9.