PMID- 8196606
OWN - NLM
STAT- MEDLINE
DCOM- 19940624
LR  - 20220317
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 14
IP  - 6
DP  - 1994 Jun
TI  - Effect of a null mutation of the insulin-like growth factor I receptor gene on 
      growth and transformation of mouse embryo fibroblasts.
PG  - 3604-12
AB  - Fibroblast cell lines, designated R- and W cells, were generated, respectively, 
      from mouse embryos homozygous for a targeted disruption of the Igf1r gene, 
      encoding the type 1 insulin-like growth factor receptor, and from their wild-type 
      littermates. W cells grow normally in serum-free medium supplemented with various 
      combinations of purified growth factors, while pre- and postcrisis R- cells 
      cannot grow, as they are arrested before entering the S phase. R- cells are able 
      to grow in 10% serum, albeit more slowly than W cells, and with all phases of the 
      cell cycle being elongated. An activated Ha-ras expressed from a stably 
      transfected plasmid is unable to overcome the inability of R- cells to grow in 
      serum-free medium supplemented with purified clones. Nevertheless, even in the 
      presence of serum, R- cells stably transfected with Ha-ras, alone or in 
      combination with simian virus 40 large T antigen, fail to form colonies in soft 
      agar. Reintroduction into R- cells (or their derivatives) of a plasmid expressing 
      the human insulin-like growth factor I receptor RNA and protein restores their 
      ability to grow with purified growth factors or in soft agar. The signaling 
      pathways participating in cell growth and transformation are discussed on the 
      basis of these results.
FAU - Sell, C
AU  - Sell C
AD  - Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, 
      Pennsylvania 19107.
FAU - Dumenil, G
AU  - Dumenil G
FAU - Deveaud, C
AU  - Deveaud C
FAU - Miura, M
AU  - Miura M
FAU - Coppola, D
AU  - Coppola D
FAU - DeAngelis, T
AU  - DeAngelis T
FAU - Rubin, R
AU  - Rubin R
FAU - Efstratiadis, A
AU  - Efstratiadis A
FAU - Baserga, R
AU  - Baserga R
LA  - eng
GR  - CA 56309/CA/NCI NIH HHS/United States
GR  - GM 33694/GM/NIGMS NIH HHS/United States
GR  - HD 28342/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Growth Substances)
RN  - 0 (Insulin)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
SB  - IM
GS  - c-Ha-ras
GS  - ras
MH  - Animals
MH  - *Cell Cycle/drug effects
MH  - Cell Division/drug effects/*physiology
MH  - Cell Line, Transformed
MH  - Cell Transformation, Neoplastic
MH  - Cells, Cultured
MH  - Embryo, Mammalian
MH  - Epidermal Growth Factor/pharmacology
MH  - Genes, ras
MH  - Growth Substances/*pharmacology
MH  - Insulin/pharmacology
MH  - Insulin-Like Growth Factor I/pharmacology
MH  - Kinetics
MH  - Mice
MH  - *Mutation
MH  - Phenotype
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Receptor, IGF Type 1/biosynthesis/*genetics
MH  - Signal Transduction
MH  - Transfection
PMC - PMC358728
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
PMCR- 1994/06/01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
PHST- 1994/06/01 00:00 [pmc-release]
AID - 10.1128/mcb.14.6.3604-3612.1994 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1994 Jun;14(6):3604-12. doi: 10.1128/mcb.14.6.3604-3612.1994.