PMID- 8269518
OWN - NLM
STAT- MEDLINE
DCOM- 19940131
LR  - 20220409
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 75
IP  - 7
DP  - 1993 Dec 31
TI  - Sonic hedgehog mediates the polarizing activity of the ZPA.
PG  - 1401-16
AB  - The zone of polarizing activity (ZPA) is a region at the posterior margin of the 
      limb bud that induces mirror-image duplications when grafted to the anterior of a 
      second limb. We have isolated a vertebrate gene, Sonic hedgehog, related to the 
      Drosophila segment polarity gene hedgehog, which is expressed specifically in the 
      ZPA and in other regions of the embryo, that is capable of polarizing limbs in 
      grafting experiments. Retinoic acid, which can convert anterior limb bud tissue 
      into tissue with polarizing activity, concomitantly induces Sonic hedgehog 
      expression in the anterior limb bud. Implanting cells that express Sonic hedgehog 
      into anterior limb buds is sufficient to cause ZPA-like limb duplications. Like 
      the ZPA, Sonic hedgehog expression leads to the activation of Hox genes. Sonic 
      hedgehog thus appears to function as the signal for antero-posterior patterning 
      in the limb.
FAU - Riddle, R D
AU  - Riddle RD
AD  - Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
FAU - Johnson, R L
AU  - Johnson RL
FAU - Laufer, E
AU  - Laufer E
FAU - Tabin, C
AU  - Tabin C
LA  - eng
SI  - GENBANK/L20888
SI  - GENBANK/L20889
SI  - GENBANK/L26081
SI  - GENBANK/L26082
SI  - GENBANK/L26083
SI  - GENBANK/L28099
SI  - GENBANK/L33709
SI  - GENBANK/S60904
SI  - GENBANK/S60905
SI  - GENBANK/S60924
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA Primers)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 149291-21-4 (hh protein, Drosophila)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chick Embryo
MH  - DNA Primers/chemistry
MH  - *Drosophila Proteins
MH  - Embryonic Induction
MH  - Extremities/*embryology
MH  - Gene Expression/drug effects
MH  - Hedgehog Proteins
MH  - In Situ Hybridization
MH  - Molecular Sequence Data
MH  - Morphogenesis
MH  - Proteins/*genetics
MH  - RNA, Messenger/genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Tretinoin/pharmacology
EDAT- 1993/12/31 00:00
MHDA- 1993/12/31 00:01
CRDT- 1993/12/31 00:00
PHST- 1993/12/31 00:00 [pubmed]
PHST- 1993/12/31 00:01 [medline]
PHST- 1993/12/31 00:00 [entrez]
AID - 0092-8674(93)90626-2 [pii]
AID - 10.1016/0092-8674(93)90626-2 [doi]
PST - ppublish
SO  - Cell. 1993 Dec 31;75(7):1401-16. doi: 10.1016/0092-8674(93)90626-2.