PMID- 8287230
OWN - NLM
STAT- MEDLINE
DCOM- 19940224
LR  - 20211203
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 2
IP  - 3
DP  - 1993 Sep
TI  - Sporadic amplification of the MYC gene in human osteosarcomas.
PG  - 163-7
AB  - The MYC proto-oncogene has been shown to be overexpressed in several types of 
      sarcomas, including some osteosarcomas. In most cases, the overexpression is due 
      to gene amplification. The total number of osteosarcoma patients studied, 
      however, remains too small to derive any conclusions regarding the true 
      prevalence and the possible clinical significance of MYC gene amplification. To 
      address the issue more thoroughly, we studied 27 specimens from 25 patients with 
      high-grade osteosarcoma (16 primary, 11 metastatic; 11 adult, 14 pediatric) for 
      MYC gene alterations by Southern blot analysis. Two of 27 specimens (7%) showed 
      MYC gene amplification: a primary fibrohistiocytic osteosarcoma of the femur in a 
      37-year-old man showed threefold amplification, and a primary Paget's 
      osteosarcoma of the tibia in a 60-year-old man showed fourfold amplification. 
      None of the specimens tested showed MYC gene rearrangement (zero of 27) or 
      activating point mutations at the PvuII site in MYC exon-1 (zero of 26). Hence, 
      the MYC gene is amplified in a subset of osteosarcomas. The possible clinical or 
      biological significance of MYC gene amplification in osteosarcoma may warrant 
      further investigation.
FAU - Ladanyi, M
AU  - Ladanyi M
AD  - Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New 
      York 10021.
FAU - Park, C K
AU  - Park CK
FAU - Lewis, R
AU  - Lewis R
FAU - Jhanwar, S C
AU  - Jhanwar SC
FAU - Healey, J H
AU  - Healey JH
FAU - Huvos, A G
AU  - Huvos AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
SB  - IM
GS  - MYC
MH  - Adult
MH  - Blotting, Southern
MH  - Bone Neoplasms/diagnosis/*genetics
MH  - Child
MH  - Female
MH  - *Gene Amplification
MH  - *Genes, myc
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Osteosarcoma/diagnosis/*genetics
MH  - Proto-Oncogene Mas
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
PST - ppublish
SO  - Diagn Mol Pathol. 1993 Sep;2(3):163-7.