PMID- 8289808
OWN - NLM
STAT- MEDLINE
DCOM- 19940224
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 14
IP  - 2
DP  - 1994 Feb
TI  - Two different types of double-strand breaks in Saccharomyces cerevisiae are 
      repaired by similar RAD52-independent, nonhomologous recombination events.
PG  - 1293-301
AB  - In haploid rad52 Saccharomyces cerevisiae strains unable to undergo homologous 
      recombination, a chromosomal double-strand break (DSB) can be repaired by 
      imprecise rejoining of the broken chromosome ends. We have used two different 
      strategies to generate broken chromosomes: (i) a site-specific DSB generated at 
      the MAT locus by HO endonuclease cutting or (ii) a random DSB generated by 
      mechanical rupture during mitotic segregation of a conditionally dicentric 
      chromosome. Broken chromosomes were repaired by deletions that were highly 
      variable in size, all of which removed more sequences than was required either to 
      prevent subsequent HO cleavage or to eliminate a functional centromere, 
      respectively. The junction of the deletions frequently occurred where 
      complementary strands from the flanking DNA could anneal to form 1 to 5 bp, 
      although 12% (4 of 34) of the events appear to have occurred by blunt-end 
      ligation. These types of deletions are very similar to the junctions observed in 
      the repair of DSBs by mammalian cells (D. B. Roth and J. H. Wilson, Mol. Cell. 
      Biol. 6:4295-4304, 1986). When a high level of HO endonuclease, expressed in all 
      phases of the cell cycle, was used to create DSBs, we also recovered a large 
      class of very small (2- or 3-bp) insertions in the HO cleavage site. These 
      insertions appear to represent still another mechanism of DSB repair, apparently 
      by annealing and filling in the overhanging 3' ends of the cleavage site. These 
      types of events have also been well documented for vertebrate cells.
FAU - Kramer, K M
AU  - Kramer KM
AD  - Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, 
      Massachusetts 02254-9110.
FAU - Brock, J A
AU  - Brock JA
FAU - Bloom, K
AU  - Bloom K
FAU - Moore, J K
AU  - Moore JK
FAU - Haber, J E
AU  - Haber JE
LA  - eng
GR  - GM07122/GM/NIGMS NIH HHS/United States
GR  - GM32238/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Fungal)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Peptides)
RN  - 0 (Pheromones)
RN  - 0 (RAD52 protein, S cerevisiae)
RN  - 0 (Rad52 DNA Repair and Recombination Protein)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 61194-02-3 (Mating Factor)
SB  - IM
GS  - MAT
GS  - RAD52
GS  - rad52
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Fungal
MH  - *DNA Damage
MH  - DNA Primers
MH  - DNA, Fungal/genetics/metabolism
MH  - DNA-Binding Proteins/biosynthesis/*genetics
MH  - Fungal Proteins/biosynthesis/*genetics
MH  - *Genes, Fungal
MH  - Mating Factor
MH  - Mitosis
MH  - Molecular Sequence Data
MH  - Mutagenesis, Insertional
MH  - Peptides/genetics
MH  - Pheromones/genetics
MH  - Polymerase Chain Reaction
MH  - Rad52 DNA Repair and Recombination Protein
MH  - *Recombination, Genetic
MH  - Saccharomyces cerevisiae/*genetics/metabolism
MH  - Saccharomyces cerevisiae Proteins
MH  - Sequence Deletion
PMC - PMC358484
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
PMCR- 1994/02/01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
PHST- 1994/02/01 00:00 [pmc-release]
AID - 10.1128/mcb.14.2.1293-1301.1994 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1994 Feb;14(2):1293-301. doi: 10.1128/mcb.14.2.1293-1301.1994.