PMID- 8295989
OWN - NLM
STAT- MEDLINE
DCOM- 19940303
LR  - 20220409
IS  - 0167-8140 (Print)
IS  - 0167-8140 (Linking)
VI  - 29
IP  - 1
DP  - 1993 Oct
TI  - Radiation-induced astrocytic and microglial responses in mouse brain.
PG  - 60-8
AB  - The aim of this study was to investigate the responses of astrocytes and 
      microglia to whole brain irradiation. Levels of glial fibrillary acidic protein 
      (GFAP), which is a marker for astrocytes, were measured by ELISA in irradiated 
      brains taken at varying time points after irradiation. GFAP levels were increased 
      between 120 and 180 days after single doses of 20-45 Gy radiation, but not after 
      lower doses (2 or 8 Gy). The increases in GFAP levels were confirmed by Western 
      blot analysis and immunohistochemical staining which showed that the number of 
      GFAP-positive astrocytes was increased, as was their staining intensity. 
      Coincidently with the increase in astrocyte staining, there was an increase in 
      the number and the intensity of microglial cell staining for Mac I antigen. 
      Autoradiography of brain tissue following in vivo administration of [3H]thymidine 
      showed an increased number of labelled cells during the same time period. The 
      radiation-induced astrocytic and microglial responses that follows brain 
      irradiation is indicative of reactive gliosis and inflammation occurring during 
      the latent period up to the onset of late radiation-induced injury. This gliosis 
      increases with radiation dose. The possibility that gliosis may participate in 
      modifying postirradiation injury in the brain is discussed.
FAU - Chiang, C S
AU  - Chiang CS
AD  - Department of Radiation Oncology, UCLA Medical Center 90024-1714.
FAU - McBride, W H
AU  - McBride WH
FAU - Withers, H R
AU  - Withers HR
LA  - eng
GR  - NCI CA-44384/CA/NCI NIH HHS/United States
GR  - NCI CA-44881/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Radiother Oncol
JT  - Radiotherapy and oncology : journal of the European Society for Therapeutic 
      Radiology and Oncology
JID - 8407192
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Macrophage-1 Antigen)
SB  - IM
MH  - Animals
MH  - Astrocytes/immunology/*radiation effects
MH  - Brain/*radiation effects
MH  - Brain Chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glial Fibrillary Acidic Protein/analysis
MH  - Macrophage-1 Antigen/analysis
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Microglia/immunology/*radiation effects
MH  - Specific Pathogen-Free Organisms
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 0167-8140(93)90174-7 [pii]
AID - 10.1016/0167-8140(93)90174-7 [doi]
PST - ppublish
SO  - Radiother Oncol. 1993 Oct;29(1):60-8. doi: 10.1016/0167-8140(93)90174-7.