PMID- 8327893
OWN - NLM
STAT- MEDLINE
DCOM- 19930809
LR  - 20190618
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 261
IP  - 5118
DP  - 1993 Jul 9
TI  - Inhibition of adenylyl cyclase by Gi alpha.
PG  - 218-21
AB  - Evidence suggests that both alpha and beta gamma subunits of heterotrimeric 
      guanine nucleotide-binding regulatory proteins (G proteins) inhibit adenylyl 
      cyclase. Although type I adenylyl cyclase is inhibited directly by exogenous beta 
      gamma, inhibition of adenylyl cyclase by Gi alpha has not been convincingly 
      demonstrated in vitro. Concentration-dependent inhibition of adenylyl cyclases by 
      purified Gi alpha subunits is described. Activated Gi alpha but not G(o) alpha 
      was effective, and myristoylation of Gi alpha was required. The characteristics 
      of the inhibitory effect were dependent on the type of adenylyl cyclase and the 
      nature of the activator of the enzyme. The concentrations of Gi alpha required to 
      inhibit adenylyl cyclase were substantially higher than those normally thought to 
      be relevant physiologically. However, analysis indicates that these 
      concentrations may be relevant and reasonable.
FAU - Taussig, R
AU  - Taussig R
AD  - Department of Pharmacology, University of Texas Southwestern Medical Center, 
      Dallas 75235-9041.
FAU - Iniguez-Lluhi, J A
AU  - Iniguez-Lluhi JA
FAU - Gilman, A G
AU  - Gilman AG
LA  - eng
GR  - GM34497/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Adenylyl Cyclase Inhibitors)
RN  - 0 (Calmodulin)
RN  - 0 (Myristic Acids)
RN  - 0 (Recombinant Proteins)
RN  - 0I3V7S25AW (Myristic Acid)
RN  - 1F7A44V6OU (Colforsin)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - *Adenylyl Cyclase Inhibitors
MH  - Adenylyl Cyclases/metabolism
MH  - Animals
MH  - Calmodulin/pharmacology
MH  - Cell Line
MH  - Colforsin/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Activation
MH  - GTP-Binding Proteins/*metabolism
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
MH  - Guanosine Triphosphate/metabolism
MH  - Moths
MH  - Myristic Acid
MH  - Myristic Acids/metabolism
MH  - Recombinant Proteins/pharmacology
EDAT- 1993/07/09 00:00
MHDA- 1993/07/09 00:01
CRDT- 1993/07/09 00:00
PHST- 1993/07/09 00:00 [pubmed]
PHST- 1993/07/09 00:01 [medline]
PHST- 1993/07/09 00:00 [entrez]
AID - 10.1126/science.8327893 [doi]
PST - ppublish
SO  - Science. 1993 Jul 9;261(5118):218-21. doi: 10.1126/science.8327893.