PMID- 8338232
OWN - NLM
STAT- MEDLINE
DCOM- 19930826
LR  - 20220311
IS  - 0003-276X (Print)
IS  - 0003-276X (Linking)
VI  - 236
IP  - 2
DP  - 1993 Jun
TI  - Dynamics of epithelial cells in the corpus of the mouse stomach. I. 
      Identification of proliferative cell types and pinpointing of the stem cell.
PG  - 259-79
AB  - In a recent study of the corpus epithelium in the mouse stomach, eleven cell 
      types have been identified and enumerated (Karam and Leblond: Anat. Rec. 
      232:231-246, 1992). The dynamics of these cells will be examined in a series of 
      five articles, of which this is the first. This article focuses on the 
      proliferative ability of the cells, as measured by the labeling index in 
      radioautographs from mice sacrificed 30 min after an intravenous injection of 
      3H-thymidine. Furthermore, the ultrastructure of the cells found to be 
      proliferative was examined in the hope of finding features characteristic of stem 
      cells. On the basis of their labeling index, the epithelial cells have been 
      classified into four groups. The first includes three cell types which do not 
      take up any label and accordingly are non-dividing: parietal or oxyntic cells, 
      cells named pre-parietal as they are immature cells suspected of being parietal 
      cell precursors, and the rare caveolated or brush cells. The second group is 
      composed of three cell types which are only rarely labeled and, therefore, divide 
      only occasionally: zymogenic or chief cells, entero-endocrine cells, and cells 
      named pre-zymogenic cells as they are suspected of being zymogenic cell 
      precursors. The third group includes two cell types which are always labeled at a 
      low degree and, therefore, divide regularly, but at a low rate: surface mucous 
      cells, herein called pit cells, whose labeling index is 0.8%, and mucous neck 
      cells, simply known as neck cells, 1.8%. The final group consists of three 
      immature cell types with high labeling indices indicating a high rate of 
      division: granule-free cells, which are devoid of secretory granules and have the 
      highest labeling index, 32.4%, pre-pit cells, which possess a few dense secretory 
      granules similar to, but smaller than, those in pit cells, 24.6%, and pre-neck 
      cells, with a small number of secretory granules similar to, but smaller than, 
      those in neck cells, 11.3%. These three cell types, as well as pre-parietal 
      cells, are rapidly renewed, with the turnover times estimated at 3.0 days for 
      pre-neck and pre-parietal cells and less than 2.6 days for granule-free and 
      pre-pit cells.(ABSTRACT TRUNCATED AT 400 WORDS)
FAU - Karam, S M
AU  - Karam SM
AD  - Department of Anatomy, McGill University, Montreal, Quebec, Canada.
FAU - Leblond, C P
AU  - Leblond CP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anat Rec
JT  - The Anatomical record
JID - 0370540
SB  - IM
MH  - Animals
MH  - Cell Division
MH  - Epithelial Cells
MH  - Epithelium/ultrastructure
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron
MH  - Parietal Cells, Gastric/*ultrastructure
MH  - Stem Cells/cytology
MH  - Stomach/*cytology
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
AID - 10.1002/ar.1092360202 [doi]
PST - ppublish
SO  - Anat Rec. 1993 Jun;236(2):259-79. doi: 10.1002/ar.1092360202.