PMID- 8342042
OWN - NLM
STAT- MEDLINE
DCOM- 19930831
LR  - 20190618
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 261
IP  - 5122
DP  - 1993 Aug 6
TI  - Selective and ATP-dependent translocation of peptides by the MHC-encoded 
      transporter.
PG  - 769-71
AB  - Major histocompatibility complex (MHC) class I molecules present peptides derived 
      from nuclear and cytosolic proteins to CD8+ T cells. These peptides are 
      translocated into the lumen of the endoplasmic reticulum (ER) to associate with 
      class I molecules. Two MHC-encoded putative transporter proteins, TAP1 and TAP2, 
      are required for efficient assembly of class I molecules and presentation of 
      endogenous peptides. Expression of TAP1 and TAP2 in a mutant cell line resulted 
      in the delivery of an 11-amino acid oligomer model peptide to the ER. Peptide 
      translocation depended on the sequence of the peptide, was adenosine triphosphate 
      (ATP)-dependent, required ATP hydrolysis, and was inhibited in a 
      concentration-dependent manner.
FAU - Neefjes, J J
AU  - Neefjes JJ
AD  - The Netherlands Cancer Institute, Amsterdam.
FAU - Momburg, F
AU  - Momburg F
FAU - Hammerling, G J
AU  - Hammerling GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 3)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Carrier Proteins)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Oligopeptides)
RN  - 0 (TAP1 protein, human)
RN  - 0 (Tap1 protein, rat)
RN  - 0 (Tap2 protein, rat)
RN  - 145892-13-3 (TAP2 protein, human)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
EIN - Science 1994 Apr 1;264(5155):16
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 3
MH  - *ATP-Binding Cassette Transporters
MH  - Adenosine Triphosphate/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biological Transport
MH  - Carrier Proteins/*metabolism
MH  - Cell Line
MH  - Cell Membrane Permeability
MH  - Endoplasmic Reticulum/metabolism
MH  - Glycosylation
MH  - Histocompatibility Antigens Class II/*metabolism
MH  - Molecular Sequence Data
MH  - Oligopeptides/*metabolism
MH  - Rats
MH  - T-Lymphocytes, Cytotoxic/*metabolism
MH  - Transfection
EDAT- 1993/08/06 00:00
MHDA- 1993/08/06 00:01
CRDT- 1993/08/06 00:00
PHST- 1993/08/06 00:00 [pubmed]
PHST- 1993/08/06 00:01 [medline]
PHST- 1993/08/06 00:00 [entrez]
AID - 10.1126/science.8342042 [doi]
PST - ppublish
SO  - Science. 1993 Aug 6;261(5122):769-71. doi: 10.1126/science.8342042.