PMID- 8426746
OWN - NLM
STAT- MEDLINE
DCOM- 19930226
LR  - 20220330
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 8
IP  - 2
DP  - 1993 Feb
TI  - Regulatory mechanism of 92 kDa type IV collagenase gene expression which is 
      associated with invasiveness of tumor cells.
PG  - 395-405
AB  - 92-kDa Type IV collagenase, a member of matrix metalloproteinases, is believed to 
      play a critical role in physiological tissue-remodeling processes and also in 
      many pathological conditions such as tumor invasion. We analyzed the 5'-flanking 
      sequence of the 92 kDa type IV collagenase gene that controls the expression of 
      the gene by ligating it to the chloramphenicol acetyltransferase gene. Deletion 
      and mutation analysis revealed that three motifs, homologous to the binding sites 
      for AP-1, NF-kappa B, and Sp-1 proteins, contributed positively to induction by 
      12-O-tetradecanoyl-phorbol-13-acetate (TPA) and tumor necrosis factor alpha (TNF 
      alpha). The AP-1 site was indispensable but not sufficient for the induction and 
      required synergistic cooperation with either the kappa B or the Sp-1 site. In OST 
      cells, a nuclear factor which bound to Sp-1 was constitutively expressed, and 
      those bound to AP-1 and kappa B elements were rapidly induced by TNF alpha 
      treatment. Comparison of the findings with those for the promoters of other 
      TPA-inducible matrix metalloproteinases, interstitial collagenase and stromelysin 
      1, revealed that the signal to the AP-1 sites is common for the TPA-inducibility 
      of the genes but that the signals to the kappa B or Sp-1 sites, which are not 
      present in interstitial collagenase and stromelysin 1 promoters, are the unique 
      determinant for the inducibility of the 92 kDa type IV collagenase gene.
FAU - Sato, H
AU  - Sato H
AD  - Department of Molecular Virology and Oncology, Kanazawa University, Ishikawa, 
      Japan.
FAU - Seiki, M
AU  - Seiki M
LA  - eng
SI  - GENBANK/D10051
PT  - Journal Article
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Base Sequence
MH  - Binding Sites
MH  - Collagenases/*genetics
MH  - DNA/metabolism
MH  - *Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Matrix Metalloproteinase 9
MH  - Molecular Sequence Data
MH  - Neoplasm Invasiveness/*genetics
MH  - Promoter Regions, Genetic
MH  - Proto-Oncogene Proteins c-jun/pharmacology
MH  - TATA Box
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transcription, Genetic
MH  - Transcriptional Activation
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1993/02/01 00:00
MHDA- 1993/02/01 00:01
CRDT- 1993/02/01 00:00
PHST- 1993/02/01 00:00 [pubmed]
PHST- 1993/02/01 00:01 [medline]
PHST- 1993/02/01 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1993 Feb;8(2):395-405.