PMID- 8497254
OWN - NLM
STAT- MEDLINE
DCOM- 19930623
LR  - 20220224
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 13
IP  - 6
DP  - 1993 Jun
TI  - Cloning and characterization of subunits of the T-cell receptor and murine 
      leukemia virus enhancer core-binding factor.
PG  - 3324-39
AB  - Moloney murine leukemia virus causes thymic leukemias when injected into newborn 
      mice. A major determinant of the thymic disease specificity of Moloney virus 
      genetically maps to the conserved viral core motif in the Moloney virus enhancer. 
      Point mutations introduced into the core site significantly shifted the disease 
      specificity of the Moloney virus from thymic leukemia to erythroid leukemia (N.A. 
      Speck, B. Renjifo, E. Golemis, T.N. Fredrickson, J.W. Hartley, and N. Hopkins, 
      Genes Dev. 4:233-242, 1990). We previously reported the purification of 
      core-binding factors (CBF) from calf thymus nuclei (S. Wang and N.A. Speck, Mol. 
      Cell. Biol. 12:89-102, 1992). CBF binds to core sites in murine leukemia virus 
      and T-cell receptor enhancers. Affinity-purified CBF contains multiple 
      polypeptides. In this study, we sequenced five tryptic peptides from two of the 
      bovine CBF proteins and isolated three cDNA clones from a mouse thymus cDNA 
      library encoding three of the tryptic peptides from the bovine proteins. The cDNA 
      clones, which we call CBF beta p22.0, CBF beta p21.5, and CBF beta p17.6, encode 
      three highly related but distinct proteins with deduced molecular sizes of 22.0, 
      21.5, and 17.6 kDa that appear to be translated from multiply spliced mRNAs 
      transcribed from the same gene. CBF beta p22.0, CBF beta p21.5, and CBF beta 
      p17.6 do not by themselves bind the core site. However, CBF beta p22.0 and CBF 
      beta p21.5 form a complex with DNA-binding CBF alpha subunits and as a result 
      decrease the rate of dissociation of the CBF protein-DNA complex. Association of 
      the CBF beta subunits does not extend the phosphate contacts in the binding site. 
      We propose that CBF beta is a non-DNA-binding subunit of CBF and does not contact 
      DNA directly.
FAU - Wang, S
AU  - Wang S
AD  - Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 
      03755.
FAU - Wang, Q
AU  - Wang Q
FAU - Crute, B E
AU  - Crute BE
FAU - Melnikova, I N
AU  - Melnikova IN
FAU - Keller, S R
AU  - Keller SR
FAU - Speck, N A
AU  - Speck NA
LA  - eng
SI  - GENBANK/L03279
SI  - GENBANK/L03305
SI  - GENBANK/L03306
GR  - R01 CA058343/CA/NCI NIH HHS/United States
GR  - CA23018/CA/NCI NIH HHS/United States
GR  - CA51065-01A1/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Core Binding Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Cell Nucleus/physiology
MH  - Chromatography, High Pressure Liquid
MH  - Cloning, Molecular/methods
MH  - Core Binding Factors
MH  - DNA/genetics/isolation & purification
MH  - DNA-Binding Proteins/*genetics/isolation & purification/metabolism
MH  - *Enhancer Elements, Genetic
MH  - Macromolecular Substances
MH  - Mice
MH  - Molecular Sequence Data
MH  - Moloney murine leukemia virus/*genetics
MH  - *Neoplasm Proteins
MH  - Oligodeoxyribonucleotides
MH  - Peptide Fragments/isolation & purification
MH  - Polymerase Chain Reaction
MH  - Protein Biosynthesis
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Antigen, T-Cell/*genetics/metabolism
MH  - T-Lymphocytes/*immunology
MH  - Thymus Gland/immunology
MH  - Transcription Factors/*genetics/isolation & purification/metabolism
MH  - Transcription, Genetic
PMC - PMC359789
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
PMCR- 1993/06/01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
PHST- 1993/06/01 00:00 [pmc-release]
AID - 10.1128/mcb.13.6.3324-3339.1993 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1993 Jun;13(6):3324-39. doi: 10.1128/mcb.13.6.3324-3339.1993.