PMID- 8602529
OWN - NLM
STAT- MEDLINE
DCOM- 19960509
LR  - 20220225
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 272
IP  - 5260
DP  - 1996 Apr 19
TI  - A mammalian histone deacetylase related to the yeast transcriptional regulator 
      Rpd3p.
PG  - 408-11
AB  - Trapoxin is a microbially derived cyclotetrapeptide that inhibits histone 
      deacetylation in vivo and causes mammalian cells to arrest in the cell cycle. A 
      trapoxin affinity matrix was used to isolate two nuclear proteins that copurified 
      with histone deacetylase activity. Both proteins were identified by peptide 
      microsequencing, and a complementary DNA encoding the histone deacetylase 
      catalytic subunit (HD1) was cloned from a human Jurkat T cell library. As the 
      predicted protein is very similar to the yeast transcriptional regulator Rpd3p, 
      these results support a role for histone deacetylase as a key regulator of 
      eukaryotic transcription.
FAU - Taunton, J
AU  - Taunton J
AD  - Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
FAU - Hassig, C A
AU  - Hassig CA
FAU - Schreiber, S L
AU  - Schreiber SL
LA  - eng
SI  - GENBANK/D31480
SI  - GENBANK/F07807
SI  - GENBANK/U50079
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fungal Proteins)
RN  - 0 (Histone Deacetylase Inhibitors)
RN  - 0 (Hydroxamic Acids)
RN  - 0 (Peptides)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - 133155-89-2 (trapoxin A)
RN  - 3X2S926L3Z (trichostatin A)
RN  - EC 3.5.1.- (RPD3 protein, S cerevisiae)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
CIN - Science. 1996 Apr 19;272(5260):371-2. PMID: 8602525
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anti-Bacterial Agents/metabolism/pharmacology
MH  - Cattle
MH  - Cell Cycle/drug effects
MH  - Cloning, Molecular
MH  - Enzyme Inhibitors/metabolism/pharmacology
MH  - Fungal Proteins/chemistry/genetics/*metabolism
MH  - *Gene Expression Regulation
MH  - Histone Deacetylase Inhibitors
MH  - Histone Deacetylases/chemistry/genetics/isolation & purification/*metabolism
MH  - Humans
MH  - Hydroxamic Acids/metabolism/pharmacology
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - *Peptides
MH  - Saccharomyces cerevisiae
MH  - Saccharomyces cerevisiae Proteins
MH  - T-Lymphocytes/enzymology
MH  - Transcription Factors/chemistry/genetics/isolation & purification/*metabolism
MH  - *Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1996/04/19 00:00
MHDA- 1996/04/19 00:01
CRDT- 1996/04/19 00:00
PHST- 1996/04/19 00:00 [pubmed]
PHST- 1996/04/19 00:01 [medline]
PHST- 1996/04/19 00:00 [entrez]
AID - 10.1126/science.272.5260.408 [doi]
PST - ppublish
SO  - Science. 1996 Apr 19;272(5260):408-11. doi: 10.1126/science.272.5260.408.