PMID- 8617954
OWN - NLM
STAT- MEDLINE
DCOM- 19960613
LR  - 20210103
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 156
IP  - 9
DP  - 1996 May 1
TI  - Immunogenicity of peptides bound to MHC class I molecules depends on the 
      MHC-peptide complex stability.
PG  - 3308-14
AB  - The impact of the MHC class I peptide binding stability on the immunogenicity of 
      particular peptide Ags in class I-restricted cytotoxic T lymphocyte responses is 
      not clearly established. Therefore, we have determined the dissociation rate of 
      each peptide from MHC class I at 37 degrees C and compared this to that of a 
      consensus CTL epitope. Newly defined immunogenic peptides formed relatively 
      stable MHC-peptide complexes as shown by their low dissociation rates, whereas 
      nonimmunogenic peptides displayed high dissociation rates. In addition virtually 
      all previously described HLA-A*0201-restricted T cell epitopes showed low 
      dissociation rates. Furthermore, we show that the immunogenicity of HIV-1-derived 
      peptides can be predicted more accurately by their dissociation rate than by the 
      MHC class I binding affinity. Selection of peptides based on affinity and their 
      dissociation rate leads to a more precise identification of candidate CTL 
      epitopes than selection based on affinity alone. These results help to understand 
      why some peptides are recognized by CTL and, along with detailed knowledge of 
      protein processing rules, therefore have important implications for the selection 
      of peptides in peptide-based vaccines.
FAU - van der Burg, S H
AU  - van der Burg SH
AD  - Department of Immunohematology and Blood Bank, University Hospital Leiden, The 
      Netherlands.
FAU - Visseren, M J
AU  - Visseren MJ
FAU - Brandt, R M
AU  - Brandt RM
FAU - Kast, W M
AU  - Kast WM
FAU - Melief, C J
AU  - Melief CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Epitopes)
RN  - 0 (Gene Products, pol)
RN  - 0 (HLA-A Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Epitopes/*chemistry/*immunology
MH  - Gene Products, pol/chemistry/immunology
MH  - HIV/chemistry/immunology
MH  - HLA-A Antigens/*chemistry/genetics/*immunology
MH  - Hepatitis B virus/chemistry/immunology
MH  - Histocompatibility Antigens Class I/*chemistry/genetics/*immunology
MH  - Humans
MH  - Kinetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Papillomaviridae/chemistry/immunology
MH  - Protein Binding/immunology
MH  - T-Lymphocytes, Cytotoxic/chemistry/immunology
EDAT- 1996/05/01 00:00
MHDA- 1996/05/01 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1996/05/01 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1996 May 1;156(9):3308-14.