PMID- 8640786
OWN - NLM
STAT- MEDLINE
DCOM- 19960715
LR  - 20071114
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 56
IP  - 5
DP  - 1996 Mar 1
TI  - Effect of radiation on interstitial fluid pressure and oxygenation in a human 
      tumor xenograft.
PG  - 964-68
AB  - Elevated interstitial fluid pressure (IFP) is a pathophysiological characteristic 
      of most human and experimental tumors and may be responsible, in part, for the 
      poor distribution of blood-borne therapeutic agents and low blood flow rate in 
      tumors. Recent data in cervical carcinomas in patients suggest that fractionated 
      radiation can lower tumor IFP and increase oxygen partial pressure (pO (2)) in 
      some patients. The goals of this study were to find the minimum dose of radiation 
      required to modulate IFP and pO(2) and to determine the time course of IFP 
      changes due to radiation in a preclinical model. Xenografts of the LS174T human 
      colon adenocarcinoma were grown in the right flank of nude (BALB/c) mice. IFP and 
      pO(2) were measured before and 24 h after graded doses of irradiation. The mean 
      +/- SD initial IFP in untreated tumors was 12.9 +/- 0.5 mm Hg (n=109), and the 
      range was 3.0 to 40.3 mm Hg. The mean +/- SD and median initial pO(2) were 20.2 
      +/- 2.4 and 11.9 mm Hg, respectively (n=37). IFP and pO(2) were independent of 
      tumor size. Fractionated radiation lowered IFP by 2.5 mm Hg when the total dose 
      was 10 or 15 Gy (P<0.05), but IFP did not change in the controls or the 5-Gy 
      radiation group (P>0.05). Irradiation increased the proportion of tumors at 
      higher oxygen tensions when compared to control tumors. The IFP and tumor volumes 
      were followed for up to 10 days after a single dose of 10, 20, or 30 Gy of 
      irradiation. IFP decreased for all treatment groups. The decrease was most 
      significant for the group receiving 30 Gy. On day five following irradiation, the 
      IFP had decreased by 35%. The changes in IFP and pO(2) occurred before any 
      macroscopic changes in tumor volume could be observed. The radiation-induced 
      decrease in IFP could be, in part, responsible for the increased uptake of 
      monoclonal antibodies following single or fractionated radiation that has been 
      reported in the literature.
FAU - Znati, C A
AU  - Znati CA
AD  - Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, 
      Pennsylvania 15213, USA.
FAU - Rosenstein, M
AU  - Rosenstein M
FAU - Boucher, Y
AU  - Boucher Y
FAU - Epperly, M W
AU  - Epperly MW
FAU - Bloomer, W D
AU  - Bloomer WD
FAU - Jain, R K
AU  - Jain RK
LA  - eng
GR  - R35-CA-56591/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
SB  - IM
MH  - Adenocarcinoma/pathology/*physiopathology/radiotherapy
MH  - Animals
MH  - Colonic Neoplasms/pathology/*physiopathology/radiotherapy
MH  - Extracellular Space/radiation effects
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Osmotic Pressure/radiation effects
MH  - Oxygen Consumption/radiation effects
MH  - Transplantation, Heterologous
EDAT- 1996/03/01 00:00
MHDA- 1996/03/01 00:01
CRDT- 1996/03/01 00:00
PHST- 1996/03/01 00:00 [pubmed]
PHST- 1996/03/01 00:01 [medline]
PHST- 1996/03/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1996 Mar 1;56(5):964-68.