PMID- 8668551
OWN - NLM
STAT- MEDLINE
DCOM- 19960805
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 24
IP  - 11
DP  - 1996 Jun 1
TI  - Regulation of in vitro gene expression using antisense oligonucleotides or 
      antisense expression plasmids transfected using starburst PAMAM dendrimers.
PG  - 2176-82
AB  - Starburst polyamidoamine (PAMAM) dendrimers are a new type of synthetic polymer 
      characterized by a branched spherical shape and a high density surface charge. We 
      have investigated the ability of these dendrimers to function as an effective 
      delivery system for antisense oligonucleotides and 'antisense expression 
      plasmids' for the targeted modulation of gene expression. Dendrimers bind to 
      various forms of nucleic acids on the basis of electrostatic interactions, and 
      the ability of DNA-dendrimer complexes to transfer oligonucleotides and plasmid 
      DNA to mediate antisense inhibition was assessed in an in vitro cell culture 
      system. Cell lines that permanently express luciferase gene were developed using 
      dendrimer mediated transfection. Transfections of antisense oligonucleotides or 
      antisense cDNA plasmids into these cell lines using dendrimers resulted in a 
      specific and dose dependent inhibition of luciferase expression. This inhibition 
      caused approximately 25-50% reduction of baseline luciferase activity. Binding of 
      the phosphodiester oligonucleotides to dendrimers also extended their 
      intracellular survival. While dendrimers were not cytotoxic at the concentrations 
      effective for DNA transfer, some non-specific suppression of luciferase 
      expression was observed. Our results indicate that Starburst dendrimers can be 
      effective carriers for the introduction of regulatory nucleic acids and 
      facilitate the suppression of the specific gene expression.
FAU - Bielinska, A
AU  - Bielinska A
AD  - Department of Internal Medicine, University of Michigan Medical School, Ann 
      Arbor, MI 48109-0666, USA.
FAU - Kukowska-Latallo, J F
AU  - Kukowska-Latallo JF
FAU - Johnson, J
AU  - Johnson J
FAU - Tomalia, D A
AU  - Tomalia DA
FAU - Baker, J R Jr
AU  - Baker JR Jr
LA  - eng
GR  - R43 CA68824/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA, Antisense)
RN  - 0 (DNA, Complementary)
RN  - 0 (Drug Carriers)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Polymers)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA, Antisense/genetics
MH  - DNA, Complementary
MH  - *Drug Carriers
MH  - Drug Stability
MH  - Electrochemistry
MH  - *Gene Expression Regulation
MH  - Luciferases/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Molecular Structure
MH  - Oligonucleotides, Antisense/*genetics
MH  - *Plasmids
MH  - *Polymers/chemistry
MH  - Rats
MH  - *Transfection
PMC - PMC145901
EDAT- 1996/06/01 00:00
MHDA- 2001/03/28 10:01
PMCR- 1996/06/01
CRDT- 1996/06/01 00:00
PHST- 1996/06/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1996/06/01 00:00 [entrez]
PHST- 1996/06/01 00:00 [pmc-release]
AID - 5g0727 [pii]
AID - 10.1093/nar/24.11.2176 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1996 Jun 1;24(11):2176-82. doi: 10.1093/nar/24.11.2176.