PMID- 8674119 OWN - NLM STAT- MEDLINE DCOM- 19960814 LR - 20210105 IS - 0092-8674 (Print) IS - 0092-8674 (Linking) VI - 85 IP - 7 DP - 1996 Jun 28 TI - The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. PG - 1135-48 AB - We examined the ability of chemokine receptors and related G protein-coupled receptors to facilitate infection by primary, clinical HIV-1 isolates. CCR5, when expressed along with CD4, the HIV-1 receptor, allowed cell lines resistant to most primary HIV-1 isolates to be infected. CCR3 facilitated infection by a more restricted subset of primary viruses, and binding of the CCR3 ligand, eotaxin, inhibited infection by these isolates. Utilization of CCR3 and CCR5 on the target cell depended upon the sequence of the third variable (V3) region of the HIV-1 gp120 exterior envelope glycoprotein. The ability of various members of the chemokine receptor family to support the early stages of HIV-1 infection helps to explain viral tropism and beta-chemokine inhibition of primary HIV-1 isolates. FAU - Choe, H AU - Choe H AD - Division of Human Retrovirology Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA. FAU - Farzan, M AU - Farzan M FAU - Sun, Y AU - Sun Y FAU - Sullivan, N AU - Sullivan N FAU - Rollins, B AU - Rollins B FAU - Ponath, P D AU - Ponath PD FAU - Wu, L AU - Wu L FAU - Mackay, C R AU - Mackay CR FAU - LaRosa, G AU - LaRosa G FAU - Newman, W AU - Newman W FAU - Gerard, N AU - Gerard N FAU - Gerard, C AU - Gerard C FAU - Sodroski, J AU - Sodroski J LA - eng GR - AI24755/AI/NIAID NIH HHS/United States GR - AI28691/AI/NIAID NIH HHS/United States GR - CA06516/CA/NCI NIH HHS/United States GR - etc. PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Cell JT - Cell JID - 0413066 RN - 0 (CCL11 protein, human) RN - 0 (CCR3 protein, human) RN - 0 (CD4 Antigens) RN - 0 (Chemokine CCL11) RN - 0 (Chemokines) RN - 0 (Chemokines, CC) RN - 0 (Cytokines) RN - 0 (Glycoproteins) RN - 0 (Receptors, CCR3) RN - 0 (Receptors, CCR5) RN - 0 (Receptors, Chemokine) RN - 0 (Receptors, Cytokine) RN - 0 (Receptors, HIV) RN - 0 (Viral Envelope Proteins) SB - IM MH - Acquired Immunodeficiency Syndrome/*virology MH - Animals MH - CD4 Antigens/physiology MH - Cell Fusion/physiology MH - Chemokine CCL11 MH - Chemokines/physiology MH - *Chemokines, CC MH - Cytokines/pharmacology MH - Dogs MH - Glycoproteins/physiology MH - HIV-1/drug effects/*physiology MH - HeLa Cells/chemistry/physiology/virology MH - Humans MH - Macrophages/chemistry/virology MH - Receptors, CCR3 MH - Receptors, CCR5 MH - *Receptors, Chemokine MH - Receptors, Cytokine/drug effects/*physiology MH - Receptors, HIV/physiology MH - T-Lymphocytes/chemistry/virology MH - Thymus Gland/cytology MH - Viral Envelope Proteins/physiology EDAT- 1996/06/28 00:00 MHDA- 1996/06/28 00:01 CRDT- 1996/06/28 00:00 PHST- 1996/06/28 00:00 [pubmed] PHST- 1996/06/28 00:01 [medline] PHST- 1996/06/28 00:00 [entrez] AID - S0092-8674(00)81313-6 [pii] AID - 10.1016/s0092-8674(00)81313-6 [doi] PST - ppublish SO - Cell. 1996 Jun 28;85(7):1135-48. doi: 10.1016/s0092-8674(00)81313-6.