PMID- 8681380
OWN - NLM
STAT- MEDLINE
DCOM- 19960816
LR  - 20191210
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 85
IP  - 6
DP  - 1996 Jun 14
TI  - An Mll-AF9 fusion gene made by homologous recombination causes acute leukemia in 
      chimeric mice: a method to create fusion oncogenes.
PG  - 853-61
AB  - Homologous recombination in embryonal stem cells has been used to produce a 
      fusion oncogene, thereby mimicking chromosomal translocations that frequently 
      result in formation of tumor-specific fusion oncogenes in human malignancies. AF9 
      sequences were fused into the mouse Mll gene so that expression of the Mll-AF9 
      fusion gene occurred from endogenous Mll transcription control elements, as in 
      t(9;11) found in human leukemias. Chimeric mice carrying the fusion gene 
      developed tumors, which were restricted to acute myeloid leukemias despite the 
      widespread activity of the Mll promoter. Onset of perceptible disease was 
      preceded by expansion of ES cell derivatives in peripheral blood. This novel use 
      of homologous recombination formally proves that chromosomal translocations 
      contribute to malignancy and provides a general strategy to create fusion 
      oncogenes for studying their role in tumorigenesis.
FAU - Corral, J
AU  - Corral J
AD  - Medical Research Council Laboratory of Molecular Biology, Cambridge, United 
      Kingdom.
FAU - Lavenir, I
AU  - Lavenir I
FAU - Impey, H
AU  - Impey H
FAU - Warren, A J
AU  - Warren AJ
FAU - Forster, A
AU  - Forster A
FAU - Larson, T A
AU  - Larson TA
FAU - Bell, S
AU  - Bell S
FAU - McKenzie, A N
AU  - McKenzie AN
FAU - King, G
AU  - King G
FAU - Rabbitts, T H
AU  - Rabbitts TH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA, Recombinant)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (MLLT3 protein, human)
RN  - 0 (Mllt3 protein, mouse)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 2.1.1.43 (Kmt2a protein, mouse)
SB  - IM
MH  - Acute Disease
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chimera
MH  - DNA, Recombinant
MH  - DNA-Binding Proteins/*genetics
MH  - Gene Targeting
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - Leukemia, Experimental/*genetics
MH  - Leukemia, Myeloid/blood/pathology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - Nuclear Proteins/*genetics
MH  - Oncogenes/*genetics
MH  - *Proto-Oncogenes
MH  - Recombinant Fusion Proteins/analysis
MH  - *Recombination, Genetic
MH  - Spleen/chemistry
MH  - Stem Cells
MH  - Thymus Gland/chemistry
MH  - *Transcription Factors
MH  - Translocation, Genetic/genetics
EDAT- 1996/06/14 00:00
MHDA- 1996/06/14 00:01
CRDT- 1996/06/14 00:00
PHST- 1996/06/14 00:00 [pubmed]
PHST- 1996/06/14 00:01 [medline]
PHST- 1996/06/14 00:00 [entrez]
AID - S0092-8674(00)81269-6 [pii]
AID - 10.1016/s0092-8674(00)81269-6 [doi]
PST - ppublish
SO  - Cell. 1996 Jun 14;85(6):853-61. doi: 10.1016/s0092-8674(00)81269-6.