PMID- 8684485
OWN - NLM
STAT- MEDLINE
DCOM- 19960819
LR  - 20220311
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 382
IP  - 6590
DP  - 1996 Aug 1
TI  - Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish.
PG  - 452-5
AB  - The notochord plays a central role in vertebrate development, acting as a 
      signalling source that patterns the neural tube and somites. In in vitro assays, 
      the secreted protein Sonic hedgehog mimics the inducing effects of notochord on 
      both presomitic mesoderm and neural plate explants of amniote embryos, suggesting 
      that both patterning activities of the notochord may be mediated by this protein 
      in vivo. In zebrafish, however, mutants with disrupted notochord development lack 
      a specific muscle cell type, the muscle pioneers, although they retain the 
      ability to induce neural differentiation, raising the possibility that neural 
      tube and somite patterning may be mediated by distinct signals. Here we describe 
      a new member of the hedgehog family, echidna hedgehog, that is expressed 
      exclusively in the notochord and has the ability to rescue the differentiation of 
      muscle pioneer cells in mutants with no notochord. Moreover, we show that a 
      combination of ectopic echidna hedgehog and sonic hedgehog expression induces 
      supernumary muscle pioneers in wild-type embryos, suggesting that both signals 
      act sequentially to pattern the developing somites.
FAU - Currie, P D
AU  - Currie PD
AD  - Molecular Embryology Laboratory, Imperial Cancer Research Fund, London, UK.
FAU - Ingham, P W
AU  - Ingham PW
LA  - eng
SI  - GENBANK/Y08426
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Cell Differentiation
MH  - *Embryonic Induction
MH  - Hedgehog Proteins
MH  - Molecular Sequence Data
MH  - Muscles/*embryology
MH  - Mutation
MH  - Notochord/*embryology/metabolism
MH  - Open Reading Frames
MH  - Phenotype
MH  - Proteins/genetics/physiology
MH  - Recombinant Fusion Proteins/pharmacology
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction
MH  - *Trans-Activators
MH  - Zebrafish
EDAT- 1996/08/01 00:00
MHDA- 1996/08/01 00:01
CRDT- 1996/08/01 00:00
PHST- 1996/08/01 00:00 [pubmed]
PHST- 1996/08/01 00:01 [medline]
PHST- 1996/08/01 00:00 [entrez]
AID - 10.1038/382452a0 [doi]
PST - ppublish
SO  - Nature. 1996 Aug 1;382(6590):452-5. doi: 10.1038/382452a0.