PMID- 8808680
OWN - NLM
STAT- MEDLINE
DCOM- 19961024
LR  - 20190914
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 5
IP  - 3
DP  - 1996 Sep
TI  - Cytotoxic T cell-resistant variants are selected in a virus-induced demyelinating 
      disease.
PG  - 253-62
AB  - C57BI/6 mice infected with mouse hepatitis virus, strain JHM (MHV-JHM) develop a 
      chronic demyelinating encephalomyelitis. Infectious virus can be isolated only 
      from symptomatic mice. In C57BI/6 mice, two CD8+ T cell epitopes within the 
      MHV-JHM surface glycoprotein were previously identified. Here, we show that 
      mutations in the RNA encoding the immunodominant of the epitopes are present in 
      nearly all virus samples isolated from these mice. Mutations are not present in 
      sequences flanking this epitope or in other CD8+ or CD4+ T cell epitopes. 
      Furthermore, analysis of five peptides corresponding to variant epitopes in 
      direct ex vivo cytotoxicity assays showed that each mutation caused a loss of 
      epitope recognition. These results suggest that escape from CD8+ T cell 
      recognition is necessary for enhanced virus replication and development of 
      clinical disease in these MHV-JHM-infected mice.
FAU - Pewe, L
AU  - Pewe L
AD  - Department of Pediatrics, University of Iowa, Iowa City 52242, USA.
FAU - Wu, G F
AU  - Wu GF
FAU - Barnett, E M
AU  - Barnett EM
FAU - Castro, R F
AU  - Castro RF
FAU - Perlman, S
AU  - Perlman S
LA  - eng
GR  - NS 24401/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Epitopes)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Coronavirus Infections/*etiology
MH  - Cytotoxicity, Immunologic
MH  - Demyelinating Diseases/*etiology
MH  - Epitopes/*genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - *Murine hepatitis virus
MH  - *Mutation
MH  - RNA, Viral/analysis
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
AID - S1074-7613(00)80320-9 [pii]
AID - 10.1016/s1074-7613(00)80320-9 [doi]
PST - ppublish
SO  - Immunity. 1996 Sep;5(3):253-62. doi: 10.1016/s1074-7613(00)80320-9.