PMID- 8816758
OWN - NLM
STAT- MEDLINE
DCOM- 19961113
LR  - 20211203
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 93
IP  - 19
DP  - 1996 Sep 17
TI  - Newly identified stress-responsive protein kinases, Krs-1 and Krs-2.
PG  - 10099-104
AB  - The activation of protein kinases is a frequent response of cells to treatment 
      with growth factors, chemicals, heat shock, or apoptosis-inducing agents. 
      However, when several agents result in the activation of the same enzymes, it is 
      unclear how specific biological responses are generated. We describe here two 
      protein kinases that are activated by a subset of stress conditions or apoptotic 
      agents but are not activated by commonly used mitogenic stimuli. Purification and 
      cloning demonstrate that these protein kinases are members of a subfamily of 
      kinases related to Ste20p, a serine/threonine kinase that functions early in a 
      pheromone responsive signal transduction cascade in yeast. The specificity of 
      Krs-1 and Krs-2 activation and their similarity to Ste20p suggest that they may 
      function at an early step in phosphorylation events that are specific responses 
      to some forms of chemical stress or extreme heat shock.
FAU - Taylor, L K
AU  - Taylor LK
AD  - Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 
      02138, USA.
FAU - Wang, H C
AU  - Wang HC
FAU - Erikson, R L
AU  - Erikson RL
LA  - eng
SI  - GENBANK/U60206
SI  - GENBANK/U60207
GR  - CA42580/CA/NCI NIH HHS/United States
GR  - CA67544/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Primers)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.7.1.- (Stk4 protein, mouse)
RN  - EC 2.7.1.11 (STK4 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (STK3 protein, human)
RN  - EC 2.7.11.1 (Serine-Threonine Kinase 3)
RN  - EC 2.7.11.1 (Stk3 protein, mouse)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinases)
RN  - EC 2.7.11.25 (STE20 protein, S cerevisiae)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Hot Temperature
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - MAP Kinase Kinase Kinases
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein Serine-Threonine Kinases/*chemistry/isolation & purification/metabolism
MH  - Recombinant Proteins/chemistry/isolation & purification/metabolism
MH  - Saccharomyces cerevisiae
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Homology, Amino Acid
MH  - Serine-Threonine Kinase 3
MH  - Signal Transduction
MH  - Software
MH  - Stress, Physiological
MH  - Transfection
PMC - PMC38343
EDAT- 1996/09/17 00:00
MHDA- 1996/09/17 00:01
PMCR- 1997/03/17
CRDT- 1996/09/17 00:00
PHST- 1996/09/17 00:00 [pubmed]
PHST- 1996/09/17 00:01 [medline]
PHST- 1996/09/17 00:00 [entrez]
PHST- 1997/03/17 00:00 [pmc-release]
AID - 10.1073/pnas.93.19.10099 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10099-104. doi: 
      10.1073/pnas.93.19.10099.