PMID- 8865208
OWN - NLM
STAT- MEDLINE
DCOM- 19970106
LR  - 20220228
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 74
IP  - 2
DP  - 1996 Sep
TI  - The potential role of dendritic cells in immune-mediated inflammatory diseases in 
      the central nervous system.
PG  - 599-608
AB  - Dendritic cells of the rat were studied immunohistochemically with MRC OX62 
      monoclonal antibody and using electron microscopy. In normal CNS, a small number 
      of OX62+ cells was detected in the choroid plexus and meninges. These cells were 
      absent from other CNS and peripheral nervous system sites studied. Dendritic 
      cells were also studied in two models of immune-mediated inflammatory conditions 
      in the CNS. These were: acute experimental allergic encephalomyelitis and 
      aberrant delayed-type hypersensitivity lesions induced as a response to 
      heat-killed bacillus Calmette-Guerin sequestrated behind the blood-brain barrier. 
      In addition, a group of animals with a delayed-type hypersensitivity response was 
      treated with dexamethasone to assess the effect of steroid treatment on T-cells 
      and OX62+ cells in CNS lesions. Dendritic cells were present in many but not all 
      lesions in acute experimental allergic encephalomyelitis and their numbers were 
      small. In experimental allergic encephalomyelitis lesions, dendritic cells were 
      found predominantly in perivascular cuffs, where they constituted approximately 
      2% of the total number of major histocompatibility complex class II+ cells. Some 
      of these cells were also detected in the CNS parenchyma, close to the 
      perivascular cuff. In contrast, dendritic cells were present in all delayed-type 
      hypersensitivity lesions studied. Their number in delayed-type hypersensitivity 
      lesions was significantly higher than in experimental allergic encephalomyelitis 
      lesions. Numerous OX62+ cells were found, even in three-month-old lesions. 
      Electron microscopy studies revealed that these cells were often in close contact 
      with lymphocytes. There was no significant change in the density of OX62+ cells, 
      IL2R+ cells and OX19+ T-cells in delayed-type hypersensitivity lesions after 
      seven-day treatment with dexamethasone, although there was a considerable 
      reduction in the number of CD45RA+ T-cells. The high numbers of dendritic cells 
      found in the delayed-type hypersensitivity lesions may be important in 
      contributing to the chronicity of the response. They may also initiate autoimmune 
      responses to CNS antigens uncovered during bystander tissue damage which occurs 
      as a consequence of aberrant delayed-type hypersensitivity responses.
FAU - Matyszak, M K
AU  - Matyszak MK
AD  - University Department of Pharmacology, University of Oxford, U.K.
FAU - Perry, V H
AU  - Perry VH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
SB  - IM
MH  - Animals
MH  - Central Nervous System Diseases/*physiopathology
MH  - Dendrites/*ultrastructure
MH  - Disease Models, Animal
MH  - Encephalomyelitis, Autoimmune, Experimental/*physiopathology
MH  - Immunohistochemistry
MH  - Inflammation/*physiopathology
MH  - Male
MH  - Microscopy, Electron
MH  - Rats
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
AID - 0306-4522(96)00160-1 [pii]
AID - 10.1016/0306-4522(96)00160-1 [doi]
PST - ppublish
SO  - Neuroscience. 1996 Sep;74(2):599-608. doi: 10.1016/0306-4522(96)00160-1.