PMID- 8878687
OWN - NLM
STAT- MEDLINE
DCOM- 19970128
LR  - 20210915
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 144
IP  - 1
DP  - 1996 Sep
TI  - Cloning of the Drosophila melanogaster meiotic recombination gene mei-218: a 
      genetic and molecular analysis of interval 15E.
PG  - 215-28
AB  - The mei-218 gene product is required for both meiotic crossing over and for the 
      production of recombination modules, suggesting that these organelles are 
      required for meiotic exchange. In this study the null phenotype of mei-218 was 
      defined through the analysis of three preexisting and five new alleles. 
      Consistent with previous studies, in homozygous mei-218 mutants meiotic crossing 
      over is reduced to < 10% of normal levels. A molecular analysis of mei-218 was 
      initiated with the isolation and mapping of lethal mutations and genome 
      rearrangements in the region containing mei-218, polytene interval 15E on the X 
      chromosome. This high resolution genetic map was aligned with a physical map 
      constructed from cosmid and P1 clones by genetically mapping restriction fragment 
      length polymorphisms and localizing rearrangement breakpoints. Within a region of 
      65 kb, we have identified seven transcription units, including mei-218 and the 
      Minute(1)15D gene, which encodes ribosomal protein S5. The mei-218 mutant 
      phenotype has been rescued by germline transformation with both a genomic 
      fragment and a cDNA under the control of the hsp83 promoter. The mei-218 gene is 
      predicted to produce an 1186-amino acid protein that has no significant 
      similarities to any known proteins.
FAU - McKim, K S
AU  - McKim KS
AD  - Section of Molecular and Cellular Biology, University of California, Davis 95616, 
      USA.
FAU - Dahmus, J B
AU  - Dahmus JB
FAU - Hawley, R S
AU  - Hawley RS
LA  - eng
SI  - GENBANK/U35631
SI  - GENBANK/U48394
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Drosophila Proteins)
RN  - 0 (mei-218 protein, Drosophila)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Cycle Proteins/*genetics
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA
MH  - DNA, Complementary
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster/*genetics
MH  - Female
MH  - Gene Deletion
MH  - Genome
MH  - Humans
MH  - Male
MH  - *Meiosis
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Phenotype
MH  - *Recombination, Genetic
MH  - Sequence Homology, Amino Acid
PMC - PMC1207495
EDAT- 1996/09/01 00:00
MHDA- 1996/09/01 00:01
PMCR- 1996/12/01
CRDT- 1996/09/01 00:00
PHST- 1996/09/01 00:00 [pubmed]
PHST- 1996/09/01 00:01 [medline]
PHST- 1996/09/01 00:00 [entrez]
PHST- 1996/12/01 00:00 [pmc-release]
AID - 1441215 [pii]
AID - 10.1093/genetics/144.1.215 [doi]
PST - ppublish
SO  - Genetics. 1996 Sep;144(1):215-28. doi: 10.1093/genetics/144.1.215.