PMID- 8887630
OWN - NLM
STAT- MEDLINE
DCOM- 19961216
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 16
IP  - 11
DP  - 1996 Nov
TI  - Transcriptional activation of the human epidermal growth factor receptor promoter 
      by human p53.
PG  - 6009-19
AB  - The human epidermal growth factor receptor (EGFR) promoter is activated by both 
      wild-type and tumor-derived mutant p53. In this communication, we demonstrate 
      that EGFR promoter sequence requirements for transactivation by wild-type and 
      mutant p53 are different. Transient-expression assays with EGFR promoter 
      deletions identified a wild-type human p53 response element, 
      5'-AGCTAGACGTCCGGGCAGCCCCCGGCG -3', from positions --265 to --239. 
      Electrophoretic mobility shift analysis and DNase I footprinting assays indicated 
      that wild-type p53 binds sequence specifically to the response element. Using 
      circularly permuted DNA fragments containing the p53-binding site, we show that 
      wild-type p53 binding induces DNA bending at this site. We further show that the 
      EGFR promoter is also activated by tumor-derived p53 mutants p53-143A, p53-175H, 
      p53-248W, p53-273H, and p53-281G. However, the transactivation by mutant p53 does 
      not require the wild-type p53-binding site. The minimal EGFR promoter from 
      positions --104 to --20 which does not contain the wild-type p53-binding site is 
      transactivated by the p53 mutants but not by the wild-type protein, showing a 
      difference in the mechanism of transactivation by wild-type and mutant p53. 
      Transactivation of the EGFR promoter by p53 may represent a novel mechanism of 
      cell growth regulation.
FAU - Ludes-Meyers, J H
AU  - Ludes-Meyers JH
AD  - Department of Microbiology, University of Texas Health Science Center at San 
      Antonio, 78284, USA.
FAU - Subler, M A
AU  - Subler MA
FAU - Shivakumar, C V
AU  - Shivakumar CV
FAU - Munoz, R M
AU  - Munoz RM
FAU - Jiang, P
AU  - Jiang P
FAU - Bigger, J E
AU  - Bigger JE
FAU - Brown, D R
AU  - Brown DR
FAU - Deb, S P
AU  - Deb SP
FAU - Deb, S
AU  - Deb S
LA  - eng
GR  - AI31498-02/AI/NIAID NIH HHS/United States
GR  - CA70712/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Composition
MH  - Base Sequence
MH  - Binding Sites
MH  - Chloramphenicol O-Acetyltransferase/biosynthesis
MH  - ErbB Receptors/*biosynthesis/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - *Promoter Regions, Genetic
MH  - Recombinant Proteins/biosynthesis
MH  - Regulatory Sequences, Nucleic Acid
MH  - Restriction Mapping
MH  - Sequence Deletion
MH  - TATA Box
MH  - *Transcriptional Activation
MH  - Tumor Suppressor Protein p53/*metabolism
PMC - PMC231603
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
PMCR- 1996/11/01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
PHST- 1996/11/01 00:00 [pmc-release]
AID - 10.1128/MCB.16.11.6009 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1996 Nov;16(11):6009-19. doi: 10.1128/MCB.16.11.6009.