PMID- 8934674
OWN - NLM
STAT- MEDLINE
DCOM- 19970124
LR  - 20190822
IS  - 0385-5600 (Print)
IS  - 0385-5600 (Linking)
VI  - 40
IP  - 3
DP  - 1996
TI  - Role of milk whey in the transmission of human cytomegalovirus infection by 
      breast milk.
PG  - 201-4
AB  - Breast-fed infants are susceptible to human cytomegalovirus (HCMV) infection via 
      breast milk. In our previous study, HCMV was isolated more frequently from breast 
      milk at later than one month after delivery than from colostrum or early breast 
      milk. To clarify the role of milk cells and whey in vertical infection by breast 
      feeding, we separated breast milk into milk cells and whey and examined each 
      fraction for the presence of HCMV. We collected breast milk from mothers who 
      breast-fed their infants (aged from 3 days to 2 months). The breast milk was 
      centrifuged and separated into the middle layer (layer of milk whey) and the 
      pellet (containing milk cells). We attempted to isolate HCMV from whey and to 
      detect HCMV immediate early (IE) DNA in both milk whey and cells. HCMV was 
      isolated from 7 out of 35 (20.0%) whey samples and HCMV IE DNA was detected from 
      15 out of 35 (42.9%) whey and/or milk cells. Detection rates of HCMV IE DNA in 
      the whey layer and milk cells were 39.1% (25 out of 64) and 17.2% (11 out of 64), 
      respectively. HCMV IE DNA was not detected in colostrum, but was detected in 
      breast milk samples one month after delivery. Therefore, cell-free HCMV shed into 
      milk whey may have a more important role in vertical infection by breast milk 
      than cell-associated HCMV in the milk.
FAU - Asanuma, H
AU  - Asanuma H
AD  - Department of Pediatrics, Sapporo Medical University, Hokkaido, Japan.
FAU - Numazaki, K
AU  - Numazaki K
FAU - Nagata, N
AU  - Nagata N
FAU - Hotsubo, T
AU  - Hotsubo T
FAU - Horino, K
AU  - Horino K
FAU - Chiba, S
AU  - Chiba S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Microbiol Immunol
JT  - Microbiology and immunology
JID - 7703966
RN  - 0 (DNA, Viral)
RN  - 0 (Milk Proteins)
SB  - IM
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cytomegalovirus/genetics/isolation & purification
MH  - Cytomegalovirus Infections/*transmission
MH  - DNA, Viral/isolation & purification
MH  - Female
MH  - Genes, Immediate-Early
MH  - Humans
MH  - Infectious Disease Transmission, Vertical
MH  - *Milk Proteins
MH  - Milk, Human/chemistry/cytology/*virology
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1111/j.1348-0421.1996.tb03335.x [doi]
PST - ppublish
SO  - Microbiol Immunol. 1996;40(3):201-4. doi: 10.1111/j.1348-0421.1996.tb03335.x.