PMID- 8940145
OWN - NLM
STAT- MEDLINE
DCOM- 19970109
LR  - 20211203
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 271
IP  - 49
DP  - 1996 Dec 6
TI  - Expression of a constitutively active Akt Ser/Thr kinase in 3T3-L1 adipocytes 
      stimulates glucose uptake and glucose transporter 4 translocation.
PG  - 31372-8
AB  - Akt is a serine/threonine kinase that requires a functional phosphatidylinositol 
      3-kinase to be stimulated by insulin and other growth factors. When directed to 
      membranes by the addition of a src myristoylation sequence, Akt becomes 
      constitutively active. In the present studies, the constitutively active Akt and 
      a nonmyristoylated control mutant were expressed in 3T3-L1 cells that can be 
      induced to differentiate into adipocytes. The constitutively active Akt induced 
      glucose uptake into adipocytes in the absence of insulin by stimulating 
      translocation of the insulin-responsive glucose transporter 4 to the plasma 
      membrane. The constitutively active Akt also increased the synthesis of the 
      ubiquitously expressed glucose transporter 1. The increased glucose influx in the 
      3T3-L1 adipocytes directed lipid but not glycogen synthesis. These results 
      indicate that Akt can regulate glucose uptake and metabolism.
FAU - Kohn, A D
AU  - Kohn AD
AD  - Department of Molecular Pharmacology, Stanford University School of Medicine, 
      Stanford, California 94305, USA. roth@cmgm.stanford.edu
FAU - Summers, S A
AU  - Summers SA
FAU - Birnbaum, M J
AU  - Birnbaum MJ
FAU - Roth, R A
AU  - Roth RA
LA  - eng
GR  - 5T32 GM07365/GM/NIGMS NIH HHS/United States
GR  - DK 34926/DK/NIDDK NIH HHS/United States
GR  - DK39615/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Glucose Transporter Type 1)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Lipids)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Myristic Acids)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Slc2a1 protein, mouse)
RN  - 0 (Slc2a4 protein, mouse)
RN  - 0I3V7S25AW (Myristic Acid)
RN  - 9005-79-2 (Glycogen)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - 3T3 Cells
MH  - Adipose Tissue/*enzymology
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Membrane
MH  - Enzyme Activation
MH  - Fibroblasts/enzymology
MH  - Glucose/*metabolism
MH  - Glucose Transporter Type 1
MH  - Glucose Transporter Type 4
MH  - Glycogen/biosynthesis
MH  - Kinetics
MH  - Lipids/biosynthesis
MH  - Mice
MH  - Monosaccharide Transport Proteins/*metabolism
MH  - *Muscle Proteins
MH  - Myristic Acid
MH  - Myristic Acids/metabolism
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-akt
EDAT- 1996/12/06 00:00
MHDA- 1996/12/06 00:01
CRDT- 1996/12/06 00:00
PHST- 1996/12/06 00:00 [pubmed]
PHST- 1996/12/06 00:01 [medline]
PHST- 1996/12/06 00:00 [entrez]
AID - S0021-9258(19)78998-4 [pii]
AID - 10.1074/jbc.271.49.31372 [doi]
PST - ppublish
SO  - J Biol Chem. 1996 Dec 6;271(49):31372-8. doi: 10.1074/jbc.271.49.31372.