PMID- 8995690
OWN - NLM
STAT- MEDLINE
DCOM- 19970218
LR  - 20201210
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 71
IP  - 2
DP  - 1997 Feb
TI  - Human cytomegalovirus infection inhibits G1/S transition.
PG  - 1629-34
AB  - Cell cycle progression during cytomegalovirus infection was investigated by 
      fluorescence-activated cell sorter (FACS) analysis of the DNA content in 
      growth-arrested as well as serum-stimulated human fibroblasts. Virus-infected 
      cells maintained in either low (0.2%) or high (10%) serum failed to progress into 
      S phase and failed to divide. DNA content analysis in the presence of G1/S 
      (hydroxyurea and mimosine) and G2/M (nocodazole and colcemid) inhibitors 
      demonstrated that upon virus infection of quiescent (G0) cells, the cell cycle 
      did not progress beyond the G1/S border even after serum stimulation. Proteins 
      which normally indicate G1/S transition (proliferating cell nuclear antigen 
      [PCNA]) or G2/M transition (cyclin B1) were elevated by virus infection. PCNA 
      levels were induced in infected cells and exhibited a punctate pattern of nuclear 
      staining instead of the diffuse pattern observed in mock-infected cells. Cyclin 
      B1 was induced in infected cells which exhibited a G1/S DNA content by FACS 
      analysis, suggesting that expression of this key cell cycle function was 
      dramatically altered by viral functions. These data demonstrate that contrary to 
      expectations, cytomegalovirus inhibits normal cell cycle progression. The host 
      cell is blocked prior to S phase to provide a favorable environment for viral 
      replication.
FAU - Dittmer, D
AU  - Dittmer D
AD  - Department of Microbiology and Immunology, Stanford University School of 
      Medicine, California 94305-5402, USA.
FAU - Mocarski, E S
AU  - Mocarski ES
LA  - eng
GR  - R01 AI20211/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Proliferating Cell Nuclear Antigen)
SB  - IM
MH  - *Cytomegalovirus
MH  - Cytomegalovirus Infections/*pathology
MH  - Fibroblasts/pathology/virology
MH  - Flow Cytometry
MH  - *G1 Phase
MH  - Humans
MH  - Proliferating Cell Nuclear Antigen/analysis
MH  - *S Phase
PMC - PMC191221
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
PMCR- 1997/02/01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
PHST- 1997/02/01 00:00 [pmc-release]
AID - 10.1128/JVI.71.2.1629-1634.1997 [doi]
PST - ppublish
SO  - J Virol. 1997 Feb;71(2):1629-34. doi: 10.1128/JVI.71.2.1629-1634.1997.